Increased expression of glutamate decarboxylase (GAD(67)) in feline lumbar spinal cord after complete thoracic spinal cord transection - PubMed (original) (raw)
Increased expression of glutamate decarboxylase (GAD(67)) in feline lumbar spinal cord after complete thoracic spinal cord transection
N J Tillakaratne et al. J Neurosci Res. 2000.
Abstract
To determine changes in gamma-aminobutyric acid (GABA) in the spinal cord in response to a complete transection, we examined the cellular and tissue changes of the two forms of GABA synthetic enzyme glutamate decarboxylase (GAD(65) and GAD(67)). In situ hybridization, immunohistochemistry, and Western blot analyses show that spinal cord transection between thoracic segments 12 and 13 results in an increase of GAD(67), but not GAD(65), protein and mRNA in the lumbar spinal cord. This increase occurs mainly in the dorsal horn and persists for at least 12 months. In addition, there was relatively high GAD(67)-immunoreactivity around the central canal, with dorsolateral GAD(67)-immunoreactive fibers extending toward the ependyma and into the central canal in the transected animals. We suggest that an increase in GAD(67) leads to increased GABA production in spinal neurons below the injury site, resulting in altered inhibition and trophic support during posttrauma recovery and adaptation. Increased GABA synthesis around the central canal, in the vicinity of ependymal cells, may represent part of a regenerative process in the mammalian spinal cord, reminiscent of that observed in lower vertebrates.
Copyright 2000 Wiley-Liss, Inc.
Similar articles
- Use-dependent modulation of inhibitory capacity in the feline lumbar spinal cord.
Tillakaratne NJ, de Leon RD, Hoang TX, Roy RR, Edgerton VR, Tobin AJ. Tillakaratne NJ, et al. J Neurosci. 2002 Apr 15;22(8):3130-43. doi: 10.1523/JNEUROSCI.22-08-03130.2002. J Neurosci. 2002. PMID: 11943816 Free PMC article. - Distribution and development of glutamic acid decarboxylase immunoreactivity in the spinal cord of the dogfish Scyliorhinus canicula (elasmobranchs).
Sueiro C, Carrera I, Molist P, Rodríguez-Moldes I, Anadón R. Sueiro C, et al. J Comp Neurol. 2004 Oct 11;478(2):189-206. doi: 10.1002/cne.20285. J Comp Neurol. 2004. PMID: 15349979 - Immunocytochemical localization of glutamate decarboxylase in rat spinal cord.
McLaughlin BJ, Barber R, Saito K, Roberts E, Wu JY. McLaughlin BJ, et al. J Comp Neurol. 1975 Dec 1;164(3):305-21. doi: 10.1002/cne.901640304. J Comp Neurol. 1975. PMID: 1184786 - Effect of injury severity on lower urinary tract function after experimental spinal cord injury.
Wrathall JR, Emch GS. Wrathall JR, et al. Prog Brain Res. 2006;152:117-34. doi: 10.1016/S0079-6123(05)52008-9. Prog Brain Res. 2006. PMID: 16198697 Review. - Can the mammalian lumbar spinal cord learn a motor task?
Hodgson JA, Roy RR, de Leon R, Dobkin B, Edgerton VR. Hodgson JA, et al. Med Sci Sports Exerc. 1994 Dec;26(12):1491-7. Med Sci Sports Exerc. 1994. PMID: 7869884 Review.
Cited by
- Magnetic Stimulation of Gigantocellular Reticular Nucleus with Iron Oxide Nanoparticles Combined Treadmill Training Enhanced Locomotor Recovery by Reorganizing Cortico-Reticulo-Spinal Circuit.
Li J, Zhou T, Wang P, Yin R, Zhang S, Cao Y, Zong L, Xiao M, Zhang Y, Liu W, Deng L, Huang F, Sun J, Wang H. Li J, et al. Int J Nanomedicine. 2024 Jul 23;19:7473-7492. doi: 10.2147/IJN.S464498. eCollection 2024. Int J Nanomedicine. 2024. PMID: 39071504 Free PMC article. - Changes in intra- and interlimb reflexes from hindlimb cutaneous afferents after staggered thoracic lateral hemisections during locomotion in cats.
Mari S, Lecomte CG, Merlet AN, Audet J, Yassine S, Eddaoui O, Genois G, Nadeau C, Harnie J, Rybak IA, Prilutsky BI, Frigon A. Mari S, et al. J Physiol. 2024 May;602(9):1987-2017. doi: 10.1113/JP286151. Epub 2024 Apr 9. J Physiol. 2024. PMID: 38593215 - Single-cell analysis reveals region-heterogeneous responses in rhesus monkey spinal cord with complete injury.
Fan Y, Wu X, Han S, Zhang Q, Sun Z, Chen B, Xue X, Zhang H, Chen Z, Yin M, Xiao Z, Zhao Y, Dai J. Fan Y, et al. Nat Commun. 2023 Aug 9;14(1):4796. doi: 10.1038/s41467-023-40513-5. Nat Commun. 2023. PMID: 37558705 Free PMC article. - Changes in synaptic inputs to dI3 INs and MNs after complete transection in adult mice.
Goltash S, Stevens SJ, Topcu E, Bui TV. Goltash S, et al. Front Neural Circuits. 2023 Jul 5;17:1176310. doi: 10.3389/fncir.2023.1176310. eCollection 2023. Front Neural Circuits. 2023. PMID: 37476398 Free PMC article. - Facilitation of sensory transmission to motoneurons during cortical or sensory-evoked primary afferent depolarization (PAD) in humans.
Metz K, Matos IC, Li Y, Afsharipour B, Thompson CK, Negro F, Quinlan KA, Bennett DJ, Gorassini MA. Metz K, et al. J Physiol. 2023 May;601(10):1897-1924. doi: 10.1113/JP284275. Epub 2023 Mar 27. J Physiol. 2023. PMID: 36916205 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical