Cytokine effects on glutamate uptake by human astrocytes - PubMed (original) (raw)
Cytokine effects on glutamate uptake by human astrocytes
S Hu et al. Neuroimmunomodulation. 2000.
Abstract
Glutamate uptake by astrocytes has been postulated to play a neuroprotective role during brain inflammation. Using primary human fetal astrocyte cultures, we investigated the influence of selected cytokines on glutamate uptake activity. Interleukin (IL)-1beta and tumor necrosis factor-alpha dose-dependently inhibited astrocyte glutamate uptake, whereas interferon (IFN)-gamma alone stimulated this activity. The nitric oxide synthase inhibitor, N(G)-monomethyl-L-arginine, blocked IL-1beta-mediated inhibition of glutamate uptake, suggesting involvement of nitric oxide in the effect of IL-1beta. IL-1 receptor antagonist protein totally reversed the inhibitory effect of cytokines, suggesting a critical role of IL-1beta. The anti-inflammatory cytokine IFN-beta blocked cytokine (IL-1beta plus IFN-gamma)-induced inhibition of glutamate uptake with a corresponding reduction in nitric oxide generation. Taken together, these findings suggest that proinflammatory cytokines inhibit astrocyte glutamate uptake by a mechanism involving nitric oxide, and that IFN-beta may exert a therapeutically beneficial effect by blocking cytokine-induced nitric oxide production in inflammatory diseases of the brain.
Copyright 2000 S. Karger AG, Basel
Similar articles
- Interleukin-1 and tumor necrosis factor-alpha synergistically mediate neurotoxicity: involvement of nitric oxide and of N-methyl-D-aspartate receptors.
Chao CC, Hu S, Ehrlich L, Peterson PK. Chao CC, et al. Brain Behav Immun. 1995 Dec;9(4):355-65. doi: 10.1006/brbi.1995.1033. Brain Behav Immun. 1995. PMID: 8903852 - Cytokine modulation of glial glutamate uptake: a possible involvement of nitric oxide.
Ye ZC, Sontheimer H. Ye ZC, et al. Neuroreport. 1996 Sep 2;7(13):2181-5. doi: 10.1097/00001756-199609020-00025. Neuroreport. 1996. PMID: 8930985 - Interleukin (IL)-1beta-mediated apoptosis of human astrocytes.
Ehrlich LC, Peterson PK, Hu S. Ehrlich LC, et al. Neuroreport. 1999 Jun 23;10(9):1849-52. doi: 10.1097/00001756-199906230-00009. Neuroreport. 1999. PMID: 10501519 - Glial glutamate transport as target for nitric oxide: consequences for neurotoxicity.
Ye ZC, Sontheimer H. Ye ZC, et al. Prog Brain Res. 1998;118:241-51. doi: 10.1016/s0079-6123(08)63212-4. Prog Brain Res. 1998. PMID: 9932446 Review.
Cited by
- Neuroinflammatory mechanisms of post-traumatic epilepsy.
Mukherjee S, Arisi GM, Mims K, Hollingsworth G, O'Neil K, Shapiro LA. Mukherjee S, et al. J Neuroinflammation. 2020 Jun 17;17(1):193. doi: 10.1186/s12974-020-01854-w. J Neuroinflammation. 2020. PMID: 32552898 Free PMC article. Review. - Cytokines and brain excitability.
Galic MA, Riazi K, Pittman QJ. Galic MA, et al. Front Neuroendocrinol. 2012 Jan;33(1):116-25. doi: 10.1016/j.yfrne.2011.12.002. Epub 2011 Dec 27. Front Neuroendocrinol. 2012. PMID: 22214786 Free PMC article. Review. - Minocycline prevents impaired glial glutamate uptake in the spinal sensory synapses of neuropathic rats.
Nie H, Zhang H, Weng HR. Nie H, et al. Neuroscience. 2010 Oct 27;170(3):901-12. doi: 10.1016/j.neuroscience.2010.07.049. Epub 2010 Aug 3. Neuroscience. 2010. PMID: 20678556 Free PMC article. - Investigating the role of interleukin-1 beta and glutamate in inflammatory bowel disease and epilepsy using discovery browsing.
Rindflesch TC, Blake CL, Cairelli MJ, Fiszman M, Zeiss CJ, Kilicoglu H. Rindflesch TC, et al. J Biomed Semantics. 2018 Dec 27;9(1):25. doi: 10.1186/s13326-018-0192-y. J Biomed Semantics. 2018. PMID: 30587224 Free PMC article. - The Key Role of Astrocytes in Amyotrophic Lateral Sclerosis and Their Commitment to Glutamate Excitotoxicity.
Provenzano F, Torazza C, Bonifacino T, Bonanno G, Milanese M. Provenzano F, et al. Int J Mol Sci. 2023 Oct 21;24(20):15430. doi: 10.3390/ijms242015430. Int J Mol Sci. 2023. PMID: 37895110 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources