The role of fractalkine in the recruitment of monocytes to the endothelium - PubMed (original) (raw)
The role of fractalkine in the recruitment of monocytes to the endothelium
G A Chapman et al. Eur J Pharmacol. 2000.
Abstract
Recombinant fractalkine possesses both chemoattractive and adhesive properties in vitro. Previous studies have demonstrated an upregulation of this molecule on the membranes of activated human endothelial cells and hypothesised that fractalkine plays a role in the recruitment and adherence of monocytes to the activated endothelium. Here we present data analysing both the adhesive and chemoattractive properties of this chemokine expressed by activated human umbilical vein endothelial cells. We demonstrate that both recombinant fractalkine and endogenously produced fractalkine function as adhesion molecules, tethering monocytes to the endothelium. However, our data demonstrate that although recombinant fractalkine has the potential to function as a potent monocyte chemoattractant, the endogenous fractalkine cleaved from activated human umbilical vein endothelial cells is not responsible for the observed chemotaxis in this model. Instead, we show that monocyte chemoattractant protein-1 (MCP-1), secreted from the activated human umbilical vein endothelial cells, is responsible for the chemotaxis of these monocytes.
Similar articles
- CX3C-chemokine, fractalkine-enhanced adhesion of THP-1 cells to endothelial cells through integrin-dependent and -independent mechanisms.
Goda S, Imai T, Yoshie O, Yoneda O, Inoue H, Nagano Y, Okazaki T, Imai H, Bloom ET, Domae N, Umehara H. Goda S, et al. J Immunol. 2000 Apr 15;164(8):4313-20. doi: 10.4049/jimmunol.164.8.4313. J Immunol. 2000. PMID: 10754331 - Fractalkine, a CX3C-chemokine, functions predominantly as an adhesion molecule in monocytic cell line THP-1.
Umehara H, Goda S, Imai T, Nagano Y, Minami Y, Tanaka Y, Okazaki T, Bloom ET, Domae N. Umehara H, et al. Immunol Cell Biol. 2001 Jun;79(3):298-302. doi: 10.1046/j.1440-1711.2001.01004.x. Immunol Cell Biol. 2001. PMID: 11380684 - Combinatorial model of chemokine involvement in glomerular monocyte recruitment: role of CXC chemokine receptor 2 in infiltration during nephrotoxic nephritis.
Zernecke A, Weber KS, Erwig LP, Kluth DC, Schröppel B, Rees AJ, Weber C. Zernecke A, et al. J Immunol. 2001 May 1;166(9):5755-62. doi: 10.4049/jimmunol.166.9.5755. J Immunol. 2001. PMID: 11313419 - Regulation of CX3CL1/fractalkine expression in endothelial cells.
Imaizumi T, Yoshida H, Satoh K. Imaizumi T, et al. J Atheroscler Thromb. 2004;11(1):15-21. doi: 10.5551/jat.11.15. J Atheroscler Thromb. 2004. PMID: 15067194 Review. - Modulation of atherogenesis by chemokines.
Boisvert WA. Boisvert WA. Trends Cardiovasc Med. 2004 May;14(4):161-5. doi: 10.1016/j.tcm.2004.02.006. Trends Cardiovasc Med. 2004. PMID: 15177267 Review.
Cited by
- Inhibition by pentoxifylline of TNF-alpha-stimulated fractalkine production in vascular smooth muscle cells: evidence for mediation by NF-kappa B down-regulation.
Chen YM, Tu CJ, Hung KY, Wu KD, Tsai TJ, Hsieh BS. Chen YM, et al. Br J Pharmacol. 2003 Mar;138(5):950-8. doi: 10.1038/sj.bjp.0705088. Br J Pharmacol. 2003. PMID: 12642397 Free PMC article. - Fractalkine/CX3CL1 enhances GABA synaptic activity at serotonin neurons in the rat dorsal raphe nucleus.
Heinisch S, Kirby LG. Heinisch S, et al. Neuroscience. 2009 Dec 15;164(3):1210-23. doi: 10.1016/j.neuroscience.2009.08.075. Epub 2009 Sep 10. Neuroscience. 2009. PMID: 19748551 Free PMC article. - Role of Fractalkine-CX3CR1 Axis in Acute Rejection of Mouse Heart Allografts Subjected to Ischemia Reperfusion Injury.
Kanzawa T, Tokita D, Saiga K, Yamakawa T, Ishigooka H, Fukuda H, Katsumata H, Miyairi S, Ishii R, Hirai T, Imai T, Okumi M, Tanabe K. Kanzawa T, et al. Transpl Int. 2022 Feb 1;35:10157. doi: 10.3389/ti.2022.10157. eCollection 2022. Transpl Int. 2022. PMID: 35185378 Free PMC article. - Fractalkine (CX3CL1) and brain inflammation: Implications for HIV-1-associated dementia.
Cotter R, Williams C, Ryan L, Erichsen D, Lopez A, Peng H, Zheng J. Cotter R, et al. J Neurovirol. 2002 Dec;8(6):585-98. doi: 10.1080/13550280290100950. J Neurovirol. 2002. PMID: 12476352 Review. - Human cytomegalovirus chemokine receptor gene US28 is transcribed in latently infected THP-1 monocytes.
Beisser PS, Laurent L, Virelizier JL, Michelson S. Beisser PS, et al. J Virol. 2001 Jul;75(13):5949-57. doi: 10.1128/JVI.75.13.5949-5957.2001. J Virol. 2001. PMID: 11390596 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous