Transcription factor S, a cleavage induction factor of the archaeal RNA polymerase - PubMed (original) (raw)
. 2000 Apr 28;275(17):12393-9.
doi: 10.1074/jbc.275.17.12393.
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- PMID: 10777522
- DOI: 10.1074/jbc.275.17.12393
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Transcription factor S, a cleavage induction factor of the archaeal RNA polymerase
W Hausner et al. J Biol Chem. 2000.
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Abstract
We have analyzed the function of an archaeal protein (now called transcription factor S (TFS)) that shows sequence similarity to eukaryotic transcription factor IIS (TFIIS) as well as to small subunits of eukaryotic RNA polymerases I (A12.6), II (B12.2), and III (C11). Western blot analysis with antibodies against recombinant TFS demonstrated that this protein is not a subunit of the RNA polymerase. In vitro transcription experiments with paused elongation complexes at position +25 showed that TFS is able to induce cleavage activity in the archaeal RNA polymerase in a similar manner to TFIIS. In the presence of TFS, the cleavage activity of the RNA polymerase truncates the RNA back to position +15 by releasing mainly dinucleotides from the 3'-end of the nascent RNA. Furthermore, TFS reduces the amount of non-chaseable elongation complexes at position +25 as well as position +45. These findings clearly demonstrate that this protein has a similar function to eukaryotic TFIIS.
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