Ca(2+)/calmodulin-dependent protein kinase II is stimulated by Wnt and Frizzled homologs and promotes ventral cell fates in Xenopus - PubMed (original) (raw)
. 2000 Apr 28;275(17):12701-11.
doi: 10.1074/jbc.275.17.12701.
Affiliations
- PMID: 10777564
- DOI: 10.1074/jbc.275.17.12701
Free article
Ca(2+)/calmodulin-dependent protein kinase II is stimulated by Wnt and Frizzled homologs and promotes ventral cell fates in Xenopus
M Kühl et al. J Biol Chem. 2000.
Free article
Abstract
Wnt ligands working through Frizzled receptors have a differential ability to stimulate release of intracellular calcium (Ca(2+)) and activation of protein kinase C (PKC). Since targets of this Ca(2+) release could play a role in Wnt signaling, we first tested the hypothesis that Ca(2+)/calmodulin-dependent protein kinase II (CamKII) is activated by some Wnt and Frizzled homologs. We report that Wnt and Frizzled homologs that activate Ca(2+) release and PKC also activate CamKII activity in Xenopus embryos, while Wnt and Frizzled homologs that activate beta-catenin function do not. This activation occurs within 10 min after receptor activation in a pertussis toxin-sensitive manner, concomitant with autophosphorylation of endogenous CamKII. Based on data that Wnt-5A and Wnt-11 are present maternally in Xenopus eggs, and activate CamKII, we then tested the hypothesis that CamKII participates in axis formation in the early embryo. Measurements of endogenous CamKII activity from dorsal and ventral regions of embryos revealed elevated activity on the prospective ventral side, which was suppressed by a dominant negative Xwnt-11. If this spatial bias in CamKII activity were involved in promoting ventral cell fate one might predict that elevating CamKII activity on the dorsal side would inhibit dorsal cell fates, while reducing CamKII activity on the ventral side would promote dorsal cell fates. Results obtained by expression of CamKII mutants were consistent with this prediction, revealing that CamKII contributes to a ventral cell fate.
Similar articles
- Protein kinase C is differentially stimulated by Wnt and Frizzled homologs in a G-protein-dependent manner.
Sheldahl LC, Park M, Malbon CC, Moon RT. Sheldahl LC, et al. Curr Biol. 1999 Jul 1;9(13):695-8. doi: 10.1016/s0960-9822(99)80310-8. Curr Biol. 1999. PMID: 10395542 - Relationship of vegetal cortical dorsal factors in the Xenopus egg with the Wnt/beta-catenin signaling pathway.
Marikawa Y, Elinson RP. Marikawa Y, et al. Mech Dev. 1999 Dec;89(1-2):93-102. doi: 10.1016/s0925-4773(99)00210-5. Mech Dev. 1999. PMID: 10559484 - The TAK1-NLK mitogen-activated protein kinase cascade functions in the Wnt-5a/Ca(2+) pathway to antagonize Wnt/beta-catenin signaling.
Ishitani T, Kishida S, Hyodo-Miura J, Ueno N, Yasuda J, Waterman M, Shibuya H, Moon RT, Ninomiya-Tsuji J, Matsumoto K. Ishitani T, et al. Mol Cell Biol. 2003 Jan;23(1):131-9. doi: 10.1128/MCB.23.1.131-139.2003. Mol Cell Biol. 2003. PMID: 12482967 Free PMC article. - New steps in the Wnt/beta-catenin signal transduction pathway.
Sakanaka C, Sun TQ, Williams LT. Sakanaka C, et al. Recent Prog Horm Res. 2000;55:225-36. Recent Prog Horm Res. 2000. PMID: 11036939 Review. - The WNT/calcium pathway: biochemical mediators, tools and future requirements.
Kühl M. Kühl M. Front Biosci. 2004 Jan 1;9:967-74. doi: 10.2741/1307. Front Biosci. 2004. PMID: 14766423 Review.
Cited by
- Unlocking Hope: Therapeutic Advances and Approaches in Modulating the Wnt Pathway for Neurodegenerative Diseases.
Faraji N, Ebadpour N, Abavisani M, Gorji A. Faraji N, et al. Mol Neurobiol. 2024 Sep 23. doi: 10.1007/s12035-024-04462-4. Online ahead of print. Mol Neurobiol. 2024. PMID: 39313658 Review. - Unraveling Cancer's Wnt Signaling: Dynamic Control through Protein Kinase Regulation.
Tümen D, Heumann P, Huber J, Hahn N, Macek C, Ernst M, Kandulski A, Kunst C, Gülow K. Tümen D, et al. Cancers (Basel). 2024 Jul 28;16(15):2686. doi: 10.3390/cancers16152686. Cancers (Basel). 2024. PMID: 39123414 Free PMC article. Review. - Unveiling the role of CaMKII in retinal degeneration: from biological mechanism to therapeutic strategies.
Sun Y, Hao M, Wu H, Zhang C, Wei D, Li S, Song Z, Tao Y. Sun Y, et al. Cell Biosci. 2024 May 9;14(1):59. doi: 10.1186/s13578-024-01236-2. Cell Biosci. 2024. PMID: 38725013 Free PMC article. Review. - Single-cell RNA-seq analysis reveals the Wnt/Ca2+ signaling pathway with inflammation, apoptosis in nucleus pulposus degeneration.
Wang P, Li Z, Ye D. Wang P, et al. BMC Musculoskelet Disord. 2024 Apr 23;25(1):321. doi: 10.1186/s12891-024-07368-3. BMC Musculoskelet Disord. 2024. PMID: 38654287 Free PMC article. - Navigating tumor angiogenesis: therapeutic perspectives and myeloid cell regulation mechanism.
Yang F, Lee G, Fan Y. Yang F, et al. Angiogenesis. 2024 Aug;27(3):333-349. doi: 10.1007/s10456-024-09913-z. Epub 2024 Apr 6. Angiogenesis. 2024. PMID: 38580870 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous