A solid-state NMR index of helical membrane protein structure and topology - PubMed (original) (raw)
A solid-state NMR index of helical membrane protein structure and topology
F M Marassi et al. J Magn Reson. 2000 May.
Abstract
The secondary structure and topology of membrane proteins can be described by inspection of two-dimensional (1)H-(15)N dipolar coupling/(15)N chemical shift polarization inversion spin exchange at the magic angle spectra obtained from uniformly (15)N-labeled samples in oriented bilayers. The characteristic wheel-like patterns of resonances observed in these spectra reflect helical wheel projections of residues in both transmembrane and in-plane helices and hence provide direct indices of the secondary structure and topology of membrane proteins in phospholipid bilayers. We refer to these patterns as PISA (polarity index slant angle) wheels. The transmembrane helix of the M2 peptide corresponding to the pore-lining segment of the acetylcholine receptor and the membrane surface helix of the antibiotic peptide magainin are used as examples.
Copyright 2000 Academic Press.
Figures
FIG. 1
PISEMA spectra calculated for a 19-residue α-helix with 3.6 residues per turn and uniform dihedral angles at various helix tilt angles relative to the bilayer normal. A. 0°. B. 10°. C. 20°. D. 30°. E. 40°. F. 50°. G. 60°. H. 70°. I. 80°. J. 90°. Spectra were calculated on a Silicon Graphics O2 computer (Mountain View, CA), using the FORTRAN program FINGERPRINT (21, 24). The principal values and molecular orientation of the 15N chemical shift tensor (σ11 = 64 ppm; σ22 = 77 ppm; σ33 = 217 ppm; σ33NH = 17°) and the NH bond distance (1.07 Å) were as previously determined (19).
FIG. 2
Helical wheel projection and two-dimensional PISEMA spectra of the uniformly 15N-labeled polypeptides in oriented lipid bilayers. A, B, and C. AChR M2. D, E, and F. Magainin II. A. Helical wheel projection of the amide N atoms of AChR M2. B. Experimental PISEMA spectrum of AChR M2, which provided most of the orientational constraints used for structure determination (10). C. Spectrum calculated for an α-helix with 3.6 residues per turn, identical dihedral angles, and a tilt of 12° relative to the membrane normal. D. Helical wheel view of the CA atoms of the magainin II helix with the N-terminus in front. The three-dimensional orientation of the peptide was determined from the angular constraints for Val17 derived from the three-dimensional solid-state NMR spectrum (24). The dashed line marks the boundary between polar (white) and hydrophobic (gray) residues in the amphipathic helix. E. Experimental PISEMA spectrum of magainin II. F. Spectrum calculated from the solution NMR structure of magainin in lipid micelles (PDB file 2MAG). Conditions for sample preparation and for solid-state NMR experiments of AChR M2 (10) and magainin (28) have been described. Spectra were calculated as described in Fig. 1.
FIG. 3
Correspondence between membrane protein helix tilt and polarity, and the resulting PISEMA spectra for uniformly 15N-labeled protein in oriented bilayers. A, D, G, and J. Helical wheels rotated by various values of the polar angle χ. B, E, H, and K. Helices rotated through various values of χ about their long axes (HA) and tilted by δ = 12° (B, E) and δ = 90° (H, K) away from the membrane normal (n). The y axis of the laboratory frame points out of the page. C, F, I, and L. Calculated PISEMA spectra for the various helix rotations and tilts. The NH bond vectors of the polar opposite residues 2 and 11 in the helical wheels are highlighted. The light gray areas in B, E, H, and K represent the lipid bilayer.
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References
- Opella SJ. NMR and membrane proteins. Nat. Struct. Biol. NMR I Suppl. 1997;4:845–848. - PubMed
- Griffin R. Dipolar recoupling in MAS spectra of biological solids. Nat. Struct. Biol. NMR II Suppl. 1998;5:508–512. - PubMed
- Smith SO, Ascheim K, Groesbeck M. Magic angle spinning NMR spectroscopy of membrane proteins. Q. Rev. Biophys. 1996;29:395–449. - PubMed
- McDowell LM, Schaefer J. High resolution NMR of biological solids. Curr. Opin. Struct. Biol. 1996;6:624–629. - PubMed
- Glaubitz C, Watts A. Magic angle-oriented sample spinning (MAOSS): A new approach toward biomembrane studies. J. Magn. Reson. 1998;130:305–316. - PubMed
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