Architecture of RNA polymerase II and implications for the transcription mechanism - PubMed (original) (raw)
Architecture of RNA polymerase II and implications for the transcription mechanism
P Cramer et al. Science. 2000.
Abstract
A backbone model of a 10-subunit yeast RNA polymerase II has been derived from x-ray diffraction data extending to 3 angstroms resolution. All 10 subunits exhibit a high degree of identity with the corresponding human proteins, and 9 of the 10 subunits are conserved among the three eukaryotic RNA polymerases I, II, and III. Notable features of the model include a pair of jaws, formed by subunits Rpb1, Rpb5, and Rpb9, that appear to grip DNA downstream of the active center. A clamp on the DNA nearer the active center, formed by Rpb1, Rpb2, and Rpb6, may be locked in the closed position by RNA, accounting for the great stability of transcribing complexes. A pore in the protein complex beneath the active center may allow entry of substrates for polymerization and exit of the transcript during proofreading and passage through pause sites in the DNA.
Comment in
- Structural biology. Light at the end of the channel.
Conaway JW, Conaway RC. Conaway JW, et al. Science. 2000 Apr 28;288(5466):632-3. doi: 10.1126/science.288.5466.632. Science. 2000. PMID: 10799002 No abstract available.
Similar articles
- Structural biology. Light at the end of the channel.
Conaway JW, Conaway RC. Conaway JW, et al. Science. 2000 Apr 28;288(5466):632-3. doi: 10.1126/science.288.5466.632. Science. 2000. PMID: 10799002 No abstract available. - Structural basis of transcription: an RNA polymerase II elongation complex at 3.3 A resolution.
Gnatt AL, Cramer P, Fu J, Bushnell DA, Kornberg RD. Gnatt AL, et al. Science. 2001 Jun 8;292(5523):1876-82. doi: 10.1126/science.1059495. Epub 2001 Apr 19. Science. 2001. PMID: 11313499 - Structural biology. A marvellous machine for making messages.
Klug A. Klug A. Science. 2001 Jun 8;292(5523):1844-6. doi: 10.1126/science.1062384. Science. 2001. PMID: 11397933 No abstract available. - Elongation by RNA polymerase II: structure-function relationship.
Gnatt A. Gnatt A. Biochim Biophys Acta. 2002 Sep 13;1577(2):175-90. doi: 10.1016/s0167-4781(02)00451-7. Biochim Biophys Acta. 2002. PMID: 12213651 Review. - Recent structural studies of RNA polymerases II and III.
Cramer P. Cramer P. Biochem Soc Trans. 2006 Dec;34(Pt 6):1058-61. doi: 10.1042/BST0341058. Biochem Soc Trans. 2006. PMID: 17073750 Review.
Cited by
- Structural basis for backtracking by the SARS-CoV-2 replication-transcription complex.
Malone B, Chen J, Wang Q, Llewellyn E, Choi YJ, Olinares PDB, Cao X, Hernandez C, Eng ET, Chait BT, Shaw DE, Landick R, Darst SA, Campbell EA. Malone B, et al. bioRxiv [Preprint]. 2021 Mar 14:2021.03.13.435256. doi: 10.1101/2021.03.13.435256. bioRxiv. 2021. PMID: 33758867 Free PMC article. Updated. Preprint. - Structural basis for backtracking by the SARS-CoV-2 replication-transcription complex.
Malone B, Chen J, Wang Q, Llewellyn E, Choi YJ, Olinares PDB, Cao X, Hernandez C, Eng ET, Chait BT, Shaw DE, Landick R, Darst SA, Campbell EA. Malone B, et al. Proc Natl Acad Sci U S A. 2021 May 11;118(19):e2102516118. doi: 10.1073/pnas.2102516118. Proc Natl Acad Sci U S A. 2021. PMID: 33883267 Free PMC article. - A two-state model for the dynamics of the pyrophosphate ion release in bacterial RNA polymerase.
Da LT, Pardo Avila F, Wang D, Huang X. Da LT, et al. PLoS Comput Biol. 2013 Apr;9(4):e1003020. doi: 10.1371/journal.pcbi.1003020. Epub 2013 Apr 4. PLoS Comput Biol. 2013. PMID: 23592966 Free PMC article. - Role of the mammalian RNA polymerase II C-terminal domain (CTD) nonconsensus repeats in CTD stability and cell proliferation.
Chapman RD, Conrad M, Eick D. Chapman RD, et al. Mol Cell Biol. 2005 Sep;25(17):7665-74. doi: 10.1128/MCB.25.17.7665-7674.2005. Mol Cell Biol. 2005. PMID: 16107713 Free PMC article. - The positions of TFIIF and TFIIE in the RNA polymerase II transcription preinitiation complex.
Chen HT, Warfield L, Hahn S. Chen HT, et al. Nat Struct Mol Biol. 2007 Aug;14(8):696-703. doi: 10.1038/nsmb1272. Epub 2007 Jul 15. Nat Struct Mol Biol. 2007. PMID: 17632521 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases