Phosphorylation of GATA-4 is involved in alpha 1-adrenergic agonist-responsive transcription of the endothelin-1 gene in cardiac myocytes - PubMed (original) (raw)
. 2000 May 5;275(18):13721-6.
doi: 10.1074/jbc.275.18.13721.
Affiliations
- PMID: 10788492
- DOI: 10.1074/jbc.275.18.13721
Free article
Phosphorylation of GATA-4 is involved in alpha 1-adrenergic agonist-responsive transcription of the endothelin-1 gene in cardiac myocytes
T Morimoto et al. J Biol Chem. 2000.
Free article
Abstract
The expression of endothelin-1 (ET-1) in cardiac myocytes is markedly induced during the development of heart failure in vivo and by stimulation with the alpha(1)-adrenergic agonist phenylephrine in culture. Although recent studies have suggested a role for cardiac-specific zinc finger GATA factors in the transcriptional pathways that modulate cardiac hypertrophy, it is unknown whether these factors are also involved in cardiac ET-1 transcription and if so, how these factors are modulated during this process. Using transient transfection assays in primary cardiac myocytes from neonatal rats, we show here that the GATA element in the rat ET-1 promoter was required for phenylephrine-stimulated ET-1 transcription. Cardiac GATA-4 bound the ET-1 GATA element and activated the ET-1 promoter in a sequence-specific manner. Stimulation by phenylephrine caused serine phosphorylation of GATA-4 and increased its ability to bind the ET-1 GATA element. Inhibition of the extracellularly responsive kinase cascade with PD098059 blocked the phenylephrine-induced increase in the DNA binding ability and the phosphorylation of GATA-4. These findings demonstrate that serine phosphorylation of GATA-4 is involved in alpha(1)-adrenergic agonist-responsive transcription of the ET-1 gene in cardiac myocytes and that extracellularly responsive kinase 1/2 activation plays a role upstream of GATA-4.
Similar articles
- Calcineurin-GATA4 pathway is involved in beta-adrenergic agonist-responsive endothelin-1 transcription in cardiac myocytes.
Morimoto T, Hasegawa K, Wada H, Kakita T, Kaburagi S, Yanazume T, Sasayama S. Morimoto T, et al. J Biol Chem. 2001 Sep 14;276(37):34983-9. doi: 10.1074/jbc.M005498200. Epub 2001 Jul 2. J Biol Chem. 2001. PMID: 11435416 - The mixed-lineage kinase 1-3 signalling pathway regulates stress response in cardiac myocytes via GATA-4 and AP-1 transcription factors.
Ola A, Kerkelä R, Tokola H, Pikkarainen S, Skoumal R, Vuolteenaho O, Ruskoaho H. Ola A, et al. Br J Pharmacol. 2010 Feb 1;159(3):717-25. doi: 10.1111/j.1476-5381.2009.00567.x. Epub 2010 Jan 8. Br J Pharmacol. 2010. PMID: 20067472 Free PMC article. - Alpha(1)-adrenergic activation of the cardiac ankyrin repeat protein gene in cardiac myocytes.
Maeda T, Sepulveda J, Chen HH, Stewart AF. Maeda T, et al. Gene. 2002 Sep 4;297(1-2):1-9. doi: 10.1016/s0378-1119(02)00924-1. Gene. 2002. PMID: 12384280 - Intracellular signaling pathways for norepinephrine- and endothelin-1-mediated regulation of myocardial cell apoptosis.
Iwai-Kanai E, Hasegawa K. Iwai-Kanai E, et al. Mol Cell Biochem. 2004 Apr;259(1-2):163-8. doi: 10.1023/b:mcbi.0000021368.80389.b9. Mol Cell Biochem. 2004. PMID: 15124920 Review. - GATA transcription factors in the developing and adult heart.
Pikkarainen S, Tokola H, Kerkelä R, Ruskoaho H. Pikkarainen S, et al. Cardiovasc Res. 2004 Aug 1;63(2):196-207. doi: 10.1016/j.cardiores.2004.03.025. Cardiovasc Res. 2004. PMID: 15249177 Review.
Cited by
- Glucose-induced cell signaling in the pathogenesis of diabetic cardiomyopathy.
Mortuza R, Chakrabarti S. Mortuza R, et al. Heart Fail Rev. 2014 Jan;19(1):75-86. doi: 10.1007/s10741-013-9381-z. Heart Fail Rev. 2014. PMID: 23430126 Review. - Endothelin-1 induces phosphorylation of GATA-4 transcription factor in the HL-1 atrial-muscle cell line.
Kitta K, Clément SA, Remeika J, Blumberg JB, Suzuki YJ. Kitta K, et al. Biochem J. 2001 Oct 15;359(Pt 2):375-80. doi: 10.1042/0264-6021:3590375. Biochem J. 2001. PMID: 11583584 Free PMC article. - Re-employment of developmental transcription factors in adult heart disease.
Oka T, Xu J, Molkentin JD. Oka T, et al. Semin Cell Dev Biol. 2007 Feb;18(1):117-31. doi: 10.1016/j.semcdb.2006.11.012. Epub 2006 Nov 24. Semin Cell Dev Biol. 2007. PMID: 17161634 Free PMC article. Review. - ZFP260 is an inducer of cardiac hypertrophy and a nuclear mediator of endothelin-1 signaling.
Komati H, Maharsy W, Beauregard J, Hayek S, Nemer M. Komati H, et al. J Biol Chem. 2011 Jan 14;286(2):1508-16. doi: 10.1074/jbc.M110.162966. Epub 2010 Nov 4. J Biol Chem. 2011. PMID: 21051538 Free PMC article. - MiR-21, MiR-29a, GATA4, and MEF2c Expression Changes in Endothelin-1 and Angiotensin II Cardiac Hypertrophy Stimulated Isl-1+Sca-1+c-kit+ Porcine Cardiac Progenitor Cells In Vitro.
Zlabinger K, Spannbauer A, Traxler D, Gugerell A, Lukovic D, Winkler J, Mester-Tonczar J, Podesser B, Gyöngyösi M. Zlabinger K, et al. Cells. 2019 Nov 9;8(11):1416. doi: 10.3390/cells8111416. Cells. 2019. PMID: 31717562 Free PMC article.