Identification of two novel TAF subunits of the yeast Saccharomyces cerevisiae TFIID complex - PubMed (original) (raw)

. 2000 May 5;275(18):13895-900.

doi: 10.1074/jbc.275.18.13895.

Affiliations

• PMID: 10788514
• DOI: 10.1074/jbc.275.18.13895

Free article

Identification of two novel TAF subunits of the yeast Saccharomyces cerevisiae TFIID complex

S L Sanders et al. J Biol Chem. 2000.

Free article

Abstract

Using a combination of ion exchange and immunoaffinity chromatography we have purified the general transcription initiation factor TFIID to near homogeneity from Saccharomyces cerevisiae. Yeast TFIID is composed of TBP, the TATA box binding protein, and 14 distinct TBP-associated factors (TAFs), which range in size from 17 to 150 kDa. Twelve of the TAF subunits have been previously identified, but two, TAF48p and TAF65p, are novel. TAF48p exhibits significant sequence similarity to the conserved C-terminal region of Drosophila TAF110p, human TAF130p, and human TAF105p and is encoded by a previously identified gene MPT1. TAF65p shows no significant sequence homology to any previously identified TAFp. The genes encoding TAF48p and TAF65p are single copy and essential for normal yeast cell growth. Furthermore, neither TAF48p nor TAF65p are associated with the histone acetylase Spt-Ada-Gcn5 complex or other non-TFIID TBF.TAF complexes. The significance of these results in terms of TFIID structure, function, and organization is discussed.

PubMed Disclaimer

Similar articles

• Molecular characterization of Saccharomyces cerevisiae TFIID.
  Sanders SL, Garbett KA, Weil PA. Sanders SL, et al. Mol Cell Biol. 2002 Aug;22(16):6000-13. doi: 10.1128/MCB.22.16.6000-6013.2002. Mol Cell Biol. 2002. PMID: 12138208 Free PMC article.
• Identification of a yeast transcription factor IID subunit, TSG2/TAF48.
  Reese JC, Zhang Z, Kurpad H. Reese JC, et al. J Biol Chem. 2000 Jun 9;275(23):17391-8. doi: 10.1074/jbc.M001635200. J Biol Chem. 2000. PMID: 10751405
• Mechanisms of transcriptional activation and repression can both involve TFIID.
  Manley JL, Um M, Li C, Ashali H. Manley JL, et al. Philos Trans R Soc Lond B Biol Sci. 1996 Apr 29;351(1339):517-26. doi: 10.1098/rstb.1996.0050. Philos Trans R Soc Lond B Biol Sci. 1996. PMID: 8735274 Review.
• The histone fold is a key structural motif of transcription factor TFIID.
  Gangloff YG, Romier C, Thuault S, Werten S, Davidson I. Gangloff YG, et al. Trends Biochem Sci. 2001 Apr;26(4):250-7. doi: 10.1016/s0968-0004(00)01741-2. Trends Biochem Sci. 2001. PMID: 11295558 Review.

Cited by

• Redundant role of tissue-selective TAF(II)105 in B lymphocytes.
  Freiman RN, Albright SR, Chu LE, Zheng S, Liang HE, Sha WC, Tjian R. Freiman RN, et al. Mol Cell Biol. 2002 Sep;22(18):6564-72. doi: 10.1128/MCB.22.18.6564-6572.2002. Mol Cell Biol. 2002. PMID: 12192054 Free PMC article.
• The coactivator dTAF(II)110/hTAF(II)135 is sufficient to recruit a polymerase complex and activate basal transcription mediated by CREB.
  Felinski EA, Quinn PG. Felinski EA, et al. Proc Natl Acad Sci U S A. 2001 Nov 6;98(23):13078-83. doi: 10.1073/pnas.241337698. Epub 2001 Oct 30. Proc Natl Acad Sci U S A. 2001. PMID: 11687654 Free PMC article.
• Core promoter binding by histone-like TAF complexes.
  Shao H, Revach M, Moshonov S, Tzuman Y, Gazit K, Albeck S, Unger T, Dikstein R. Shao H, et al. Mol Cell Biol. 2005 Jan;25(1):206-19. doi: 10.1128/MCB.25.1.206-219.2005. Mol Cell Biol. 2005. PMID: 15601843 Free PMC article.
• Distinct mutations in yeast TAF(II)25 differentially affect the composition of TFIID and SAGA complexes as well as global gene expression patterns.
  Kirschner DB, vom Baur E, Thibault C, Sanders SL, Gangloff YG, Davidson I, Weil PA, Tora L. Kirschner DB, et al. Mol Cell Biol. 2002 May;22(9):3178-93. doi: 10.1128/MCB.22.9.3178-3193.2002. Mol Cell Biol. 2002. PMID: 11940675 Free PMC article.
• Molecular characterization of Saccharomyces cerevisiae TFIID.
  Sanders SL, Garbett KA, Weil PA. Sanders SL, et al. Mol Cell Biol. 2002 Aug;22(16):6000-13. doi: 10.1128/MCB.22.16.6000-6013.2002. Mol Cell Biol. 2002. PMID: 12138208 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • BioCyc
  • Gene Ontology
  • Saccharomyces Genome Database

• Research Materials

  • NCI CPTC Antibody Characterization Program