A counterselection for the tryptophan pathway in yeast: 5-fluoroanthranilic acid resistance - PubMed (original) (raw)
A counterselection for the tryptophan pathway in yeast: 5-fluoroanthranilic acid resistance
J H Toyn et al. Yeast. 2000 Apr.
Abstract
The ability to counterselect, as well as to select for, a genetic marker has numerous applications in microbial genetics. Described here is the use of 5-fluoroanthranilic acid for the counterselection of TRP1, a commonly used genetic marker in the yeast Saccharomyces cerevisiae. Counterselection using 5-fluoroanthranilic acid involves antimetabolism by the enzymes of the tryptophan biosynthetic pathway, such that trp1, trp3, trp4 or trp5 strains, which lack enzymes required for the conversion of anthranilic acid to tryptophan, are resistant to 5-fluoroanthranilic acid. Commonly used genetic procedures, such as selection for loss of a chromosomally integrated plasmid, and a replica-plating method to rapidly assess genetic linkage in self-replicating shuttle vectors, can now be carried out using the TRP1 marker gene. In addition, novel tryptophan auxotrophs can be selected using 5-fluoroanthranilic acid.
Copyright 2000 John Wiley & Sons, Ltd.
Similar articles
- The spectrum of Trp- mutants isolated as 5-fluoroanthranilate-resistant clones in Saccharomyces bayanus, S. mikatae and S. paradoxus.
Jones EW, Berget PB, Burnette JM 3rd, Anderson C, Asafu-Adjei D, Avetisian S, Barrie F, Chen R, Chu B, Conroy S, Conroy S, Dill A, Eimer W, Garrity D, Greenwood A, Hamilton T, Hucko S, Jackson C, Livesey K, Monaco T, Onorato C, Otsuka M, Pai S, Schaeffer G, Shung S, Spath S, Stahlman J, Sweeney B, Wiltrout E, Yurovsky D, Zonneveld A. Jones EW, et al. Yeast. 2008 Jan;25(1):41-6. doi: 10.1002/yea.1552. Yeast. 2008. PMID: 17924454 - Isolation and characterization of the TRP1 gene from the yeast Yarrowia lipolytica and multiple gene disruption using a TRP blaster.
Cheon SA, Han EJ, Kang HA, Ogrydziak DM, Kim JY. Cheon SA, et al. Yeast. 2003 Jun;20(8):677-85. doi: 10.1002/yea.987. Yeast. 2003. PMID: 12794929 - Selective fitness of four episomal shuttle-vectors carrying HIS3, LEU2, TRP1, and URA3 selectable markers in Saccharomyces cerevisiae.
Ugolini S, Tosato V, Bruschi CV. Ugolini S, et al. Plasmid. 2002 Mar;47(2):94-107. doi: 10.1006/plas.2001.1557. Plasmid. 2002. PMID: 11982331 - Evolution of a biosynthetic pathway: the tryptophan paradigm.
Crawford IP. Crawford IP. Annu Rev Microbiol. 1989;43:567-600. doi: 10.1146/annurev.mi.43.100189.003031. Annu Rev Microbiol. 1989. PMID: 2679363 Review. No abstract available. - [Protein-protein interactions in tryptophan biosynthetic enzymes: tryptophan synthetase and anthranilate synthetase (author's transl)].
Ito J. Ito J. Tanpakushitsu Kakusan Koso. 1973 Jun;18(5):462-71. Tanpakushitsu Kakusan Koso. 1973. PMID: 4582322 Review. Japanese. No abstract available.
Cited by
- Evolution of a Restriction Factor by Domestication of a Yeast Retrotransposon.
Hannon-Hatfield JA, Chen J, Bergman CM, Garfinkel DJ. Hannon-Hatfield JA, et al. Mol Biol Evol. 2024 Mar 1;41(3):msae050. doi: 10.1093/molbev/msae050. Mol Biol Evol. 2024. PMID: 38442736 Free PMC article. - The synthetic future of algal genomes.
Goold HD, Moseley JL, Lauersen KJ. Goold HD, et al. Cell Genom. 2024 Mar 13;4(3):100505. doi: 10.1016/j.xgen.2024.100505. Epub 2024 Feb 22. Cell Genom. 2024. PMID: 38395701 Free PMC article. Review. - Signification and Application of Mutator and Antimutator Phenotype-Induced Genetic Variations in Evolutionary Adaptation and Cancer Therapeutics.
Chung WH. Chung WH. J Microbiol. 2023 Dec;61(12):1013-1024. doi: 10.1007/s12275-023-00091-z. Epub 2023 Dec 15. J Microbiol. 2023. PMID: 38100001 Review. - Cornelia de Lange Syndrome mutations in SMC1A cause cohesion defects in yeast.
Chen J, Floyd EN, Dawson DS, Rankin S. Chen J, et al. Genetics. 2023 Oct 4;225(2):iyad159. doi: 10.1093/genetics/iyad159. Genetics. 2023. PMID: 37650609 Free PMC article. - Practical Approaches for the Yeast Saccharomyces cerevisiae Genome Modification.
Stepchenkova EI, Zadorsky SP, Shumega AR, Aksenova AY. Stepchenkova EI, et al. Int J Mol Sci. 2023 Jul 26;24(15):11960. doi: 10.3390/ijms241511960. Int J Mol Sci. 2023. PMID: 37569333 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases