Method for screening peptide fragment ion mass spectra prior to database searching - PubMed (original) (raw)

Method for screening peptide fragment ion mass spectra prior to database searching

R E Moore et al. J Am Soc Mass Spectrom. 2000 May.

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Abstract

A methodology is described for screening fragment ion spectra of peptides prior to database searching for protein identification. A software routine written in the Perl programming language was used to analyze data from previous Sequest database searches and develop a set of statistical descriptors that could be used to identify spectra not likely to yield useful results in a database search. A second Perl program used an evolutionary algorithm to optimize the criteria for each statistical descriptor and generate a formula for determining spectral quality. This formula was used by a third Perl program to screen data sets from four independent liquid chromatography tandem mass spectrometry runs. On the average, use of the screening program reduced the time required for a database search by 1/2 with little loss of useful information from the database search results.

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References

    1. Anal Chem. 1994 Dec 15;66(24):4390-9 - PubMed
    1. Biochem Biophys Res Commun. 1993 Aug 31;195(1):58-64 - PubMed
    1. Curr Biol. 1993 Jun 1;3(6):327-32 - PubMed
    1. Protein Sci. 1998 Mar;7(3):706-19 - PubMed
    1. Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):5011-5 - PubMed

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