The Toxoplasma adhesive protein MIC2 is proteolytically processed at multiple sites by two parasite-derived proteases - PubMed (original) (raw)
. 2000 May 12;275(19):14346-53.
doi: 10.1074/jbc.275.19.14346.
Affiliations
- PMID: 10799515
- DOI: 10.1074/jbc.275.19.14346
Free article
The Toxoplasma adhesive protein MIC2 is proteolytically processed at multiple sites by two parasite-derived proteases
V B Carruthers et al. J Biol Chem. 2000.
Free article
Abstract
MIC2 is an adhesive protein that participates in host cell invasion by the obligate intracellular parasite Toxoplasma gondii. Earlier studies established that MIC2 is secreted into the culture medium by extracellular parasites and that release is coincident with proteolytic modification. Since little is known about proteolytic processing of proteins secreted by T. gondii, we undertook this study to investigate the proteolytic events that accompany secretion of MIC2. We demonstrate that the C-terminal domain of MIC2 is removed by a protease, termed MPP1, when MIC2 is released into the culture supernatant. Additionally, prior to release, a second protease, termed MPP2, trims the N terminus of MIC2, resulting in the release of heterogeneously sized species of MIC2. Although MPP1 activity was unaffected by any of the protease inhibitors tested, MPP2 activity was blocked by a subset of serine and cysteine protease inhibitors. These results establish that MIC2 is proteolytically modified at multiple sites by two distinct enzymes that probably operate on the parasite surface.
Similar articles
- Proteomic analysis of cleavage events reveals a dynamic two-step mechanism for proteolysis of a key parasite adhesive complex.
Zhou XW, Blackman MJ, Howell SA, Carruthers VB. Zhou XW, et al. Mol Cell Proteomics. 2004 Jun;3(6):565-76. doi: 10.1074/mcp.M300123-MCP200. Epub 2004 Feb 24. Mol Cell Proteomics. 2004. PMID: 14982962 - C-terminal processing of the toxoplasma protein MIC2 is essential for invasion into host cells.
Brossier F, Jewett TJ, Lovett JL, Sibley LD. Brossier F, et al. J Biol Chem. 2003 Feb 21;278(8):6229-34. doi: 10.1074/jbc.M209837200. Epub 2002 Dec 5. J Biol Chem. 2003. PMID: 12471033 - Multimerization of the Toxoplasma gondii MIC2 integrin-like A-domain is required for binding to heparin and human cells.
Harper JM, Hoff EF, Carruthers VB. Harper JM, et al. Mol Biochem Parasitol. 2004 Apr;134(2):201-12. doi: 10.1016/j.molbiopara.2003.12.001. Mol Biochem Parasitol. 2004. PMID: 15003840 - Toxoplasma gondii: microneme protein MIC2.
Brossier F, David Sibley L. Brossier F, et al. Int J Biochem Cell Biol. 2005 Nov;37(11):2266-72. doi: 10.1016/j.biocel.2005.06.006. Int J Biochem Cell Biol. 2005. PMID: 16084754 Review. - Preparing for an invasion: charting the pathway of adhesion proteins to Toxoplasma micronemes.
Huynh MH, Harper JM, Carruthers VB. Huynh MH, et al. Parasitol Res. 2006 Apr;98(5):389-95. doi: 10.1007/s00436-005-0062-2. Epub 2005 Dec 30. Parasitol Res. 2006. PMID: 16385407 Review.
Cited by
- TBC9, an essential TBC-domain protein, regulates early vesicular transport and IMC formation in Toxoplasma gondii.
Sun M, Tang T, He K, Long S. Sun M, et al. Commun Biol. 2024 May 18;7(1):596. doi: 10.1038/s42003-024-06310-6. Commun Biol. 2024. PMID: 38762629 Free PMC article. - Analysis of CDPK1 targets identifies a trafficking adaptor complex that regulates microneme exocytosis in Toxoplasma.
Chan AW, Broncel M, Yifrach E, Haseley NR, Chakladar S, Andree E, Herneisen AL, Shortt E, Treeck M, Lourido S. Chan AW, et al. Elife. 2023 Nov 7;12:RP85654. doi: 10.7554/eLife.85654. Elife. 2023. PMID: 37933960 Free PMC article. - The cortical microtubules of Toxoplasma gondii underlie the helicity of parasite movement.
Tengganu IF, Arias Padilla LF, Munera Lopez J, Liu J, Brown PT, Murray JM, Hu K. Tengganu IF, et al. J Cell Sci. 2023 Sep 1;136(17):jcs261270. doi: 10.1242/jcs.261270. Epub 2023 Sep 7. J Cell Sci. 2023. PMID: 37675776 Free PMC article. - Functional screening reveals Toxoplasma prenylated proteins required for endocytic trafficking and rhoptry protein sorting.
Wang QQ, Sun M, Tang T, Lai DH, Liu J, Maity S, He K, Wu XT, Yang J, Li YB, Tang XY, Ding HY, Hide G, Distefano M, Lun ZR, Zhu XQ, Long S. Wang QQ, et al. mBio. 2023 Aug 31;14(4):e0130923. doi: 10.1128/mbio.01309-23. Epub 2023 Aug 7. mBio. 2023. PMID: 37548452 Free PMC article. - Analysis of CDPK1 targets identifies a trafficking adaptor complex that regulates microneme exocytosis in Toxoplasma.
Chan AW, Broncel M, Yifrach E, Haseley N, Chakladar S, Andree E, Herneisen AL, Shortt E, Treeck M, Lourido S. Chan AW, et al. bioRxiv [Preprint]. 2023 Aug 29:2023.01.11.523553. doi: 10.1101/2023.01.11.523553. bioRxiv. 2023. PMID: 36712004 Free PMC article. Updated. Preprint.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous