Flowcell culture of Porphyromonas gingivalis biofilms under anaerobic conditions - PubMed (original) (raw)

Flowcell culture of Porphyromonas gingivalis biofilms under anaerobic conditions

M C Hansen et al. J Microbiol Methods. 2000 May.

Abstract

We have developed an anaerobic biofilm culture system. The system is inexpensive, simple to use and, unlike an anaerobic glovebox, requires no dedicated space. As a test of the system, Porphyromonas gingivalis was cultured under low oxygen (1-2 ppm) and under anaerobic conditions (</=0.1 ppm O(2)). In the presence of small amounts of oxygen, the organism attached and formed an initial biofilm over the course of 4 h, but the biofilm was unable to maintain its growth and had lost biomass after 18 h. Also, ambiguous results were obtained when the biofilm was stained with a viability stain. Under anaerobic conditions, the biofilm was able to continue growth - biomass was greater after 18 h than after 4 h, and the anaerobic biofilm had a less ambiguous staining pattern than did the low-O(2)-grown biofilm.

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