Th2 cell membrane factors in association with IL-4 enhance matrix metalloproteinase-1 (MMP-1) while decreasing MMP-9 production by granulocyte-macrophage colony-stimulating factor-differentiated human monocytes - PubMed (original) (raw)

Th2 cell membrane factors in association with IL-4 enhance matrix metalloproteinase-1 (MMP-1) while decreasing MMP-9 production by granulocyte-macrophage colony-stimulating factor-differentiated human monocytes

C Chizzolini et al. J Immunol. 2000.

Abstract

Monocytes/macrophages are directly involved in tissue remodeling and tissue destruction through the release of matrix metalloproteinases (MMP). In the present study, we examined the effect mediated by contact of polarized Th cells with mononuclear phagocytes on the production of MMP-1, MMP-9, and their inhibitor. Plasma cell membranes from Ag-activated Th1 and Th2 cells were potent inducers of MMP-1 production by THP-1 cells. Cell membrane-associated TNF was found to be only partially involved in MMP-1 induction by both Th1 and Th2 cells. In Th2 cells exclusively, membrane-associated IL-4 induced MMP-1 production by THP-1 cells. This membrane-associated IL-4 effect was additive to that of TNF and was specifically observed on MMP-1 as MMP-9 production was concomitantly inhibited. Similarly, soluble IL-4 induced THP-1 cells to produce MMP-1, its effect proving additive to that of soluble TNF and to that of cell membranes of mitogen-activated HUT-78 cells. Its activity was blocked by IL-4 neutralization, and was unaffected by the presence of indomethacin. These effects on THP-1 cells were observed at protein and mRNA levels. Although inhibitory on freshly isolated peripheral blood monocytes, soluble IL-4 enhanced T cell-induced MMP-1 and inhibited MMP-9 production both at protein and mRNA levels in monocytes cultured for 7 days in the presence of GM-CSF. Thus, in contrast with previously reported effects, Th2 and IL-4 specifically induce MMP-1 production by mononuclear phagocytes at various stages of differentiation. This IL-4 activity may be relevant to pathological conditions dominated by Th2 inflammatory responses, resulting in tissue remodeling and destruction.

PubMed Disclaimer

Cited by

Roles of differently polarized macrophages in the initiation and progressionof pancreatic cancer.
Storz P. Storz P. Front Immunol. 2023 Aug 11;14:1237711. doi: 10.3389/fimmu.2023.1237711. eCollection 2023. Front Immunol. 2023. PMID: 37638028 Free PMC article. Review.
Mechanisms and Effects of Macrophage Polarization and Its Specifics in Pulmonary Environment.
Žaloudíková M. Žaloudíková M. Physiol Res. 2023 Jul 31;72(Suppl 2):S137-S156. doi: 10.33549/physiolres.935058. Physiol Res. 2023. PMID: 37565418 Free PMC article. Review.
A review of current status of cell-based therapies for aortic aneurysms.
Yamawaki-Ogata A, Mutsuga M, Narita Y. Yamawaki-Ogata A, et al. Inflamm Regen. 2023 Aug 7;43(1):40. doi: 10.1186/s41232-023-00280-8. Inflamm Regen. 2023. PMID: 37544997 Free PMC article. Review.
Mitochondrial DNA on Tumor-Associated Macrophages Polarization and Immunity.
Guo Y, Tsai HI, Zhang L, Zhu H. Guo Y, et al. Cancers (Basel). 2022 Mar 11;14(6):1452. doi: 10.3390/cancers14061452. Cancers (Basel). 2022. PMID: 35326602 Free PMC article. Review.
The genomic architecture of metastasis in breast cancer: focus on mechanistic aspects, signalling pathways and therapeutic strategies.
Chhichholiya Y, Suman P, Singh S, Munshi A. Chhichholiya Y, et al. Med Oncol. 2021 Jul 16;38(8):95. doi: 10.1007/s12032-021-01547-1. Med Oncol. 2021. PMID: 34268641 Review.

Th2 cell membrane factors in association with IL-4 enhance matrix metalloproteinase-1 (MMP-1) while decreasing MMP-9 production by granulocyte-macrophage colony-stimulating factor-differentiated human monocytes - PubMed (original) (raw)