Bcl-2 decreases the free Ca2+ concentration within the endoplasmic reticulum - PubMed (original) (raw)

Bcl-2 decreases the free Ca2+ concentration within the endoplasmic reticulum

R Foyouzi-Youssefi et al. Proc Natl Acad Sci U S A. 2000.

Abstract

The antiapoptotic protein Bcl-2 localizes not only to mitochondria but also to the endoplasmic reticulum (ER). However, the function of Bcl-2 at the level of the ER is poorly understood. In this study, we have investigated the effects of Bcl-2 expression on Ca(2+) storage and release by the ER. The expression of Bcl-2 decreased the amount of Ca(2+) that could be released from intracellular stores, regardless of the mode of store depletion, the cell type, or the species from which Bcl-2 was derived. Bcl-2 also decreased cellular Ca(2+) store content in the presence of mitochondrial inhibitors, suggesting that its effects were not mediated through mitochondrial Ca(2+) uptake. Direct measurements with ER-targeted Ca(2+)-sensitive fluorescent "cameleon" proteins revealed that Bcl-2 decreased the free Ca(2+) concentration within the lumen of the ER, [Ca(2+)](ER). Analysis of the kinetics of Ca(2+) store depletion in response to the Ca(2+)-ATPase inhibitor thapsigargin revealed that Bcl-2 increased the permeability of the ER membrane. These results suggest that Bcl-2 decreases the free Ca(2+) concentration within the ER lumen by increasing the Ca(2+) permeability of the ER membrane. The increased ER Ca(2+) permeability conferred by Bcl-2 would be compatible with an ion channel function of Bcl-2 at the level of the ER membrane.

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Figures

Figure 1

Figure 1

Western blot and immunofluorescence analysis of Bcl-2 expression in transfected cells. Western blot of control, mBcl-2, and hBcl-2-expressing R6 rat embryo fibroblasts (A) or HEK-293 cells (B). (C–F) Fluorescence imaging analysis of Bcl-2 expression in HEK-293 cells, transiently cotransfected with the hBcl-2 (or control vector) and with ER-targeted cameleon (Cam4-ER). (C) Cam4-ER fluorescence. (D) Bcl-2 immunofluorescence. (E) Pseudocolored and merged images of the cells (yellow = colocalization). (F) Differential interference contrast Nomarski image of the cells.

Figure 2

Figure 2

Bcl-2 reduces the amount of releasable Ca2+ from intracellular stores. (A) [Ca2+]c trace in control and Bcl-2-expressing A20 cells in response to 100 nM TG. (B) Peak [Ca2+]c increase in response to 100 nM TG in control and Bcl-2-expressing A20 cells. (C) [Ca2+]c trace in control and Bcl-2-expressing A20 cells in response to 100 nM ionomycin. (D) Peak [Ca2+]c increase in response to 100 nM ionomycin in control and Bcl-2-expressing A20 cells. (E) [Ca2+]c traces in control R6 cells and R6 cells expressing hBcl-2 or mBcl-2, in response to 100 nM TG. (F) Peak Ca2+ increase in response to 100 nM TG in control R6 cells and R6 cells expressing hBcl-2 or mBcl-2 (the initial short-lasting upstroke seen in R6-hBcl-2 cells is pipetting artifact). Data shown in B, D, and F are mean ± SEM from four independent experiments.

Figure 3

Figure 3

Bcl-2 reduces the free Ca2+ concentration within the ER. (A) Measurements using ER-targeted cameleon. (Upper) Single wavelength cameleon fluorescence (emission 535 nm) of control and hBcl-2-expressing HEK-293 cells, showing the reticular pattern typical of the ER. (Lower) Corresponding emission ratio images of the same cells (535/475 nm), thresholded to extract the region of interest that were spatially averaged to yield [Ca2+]ER values. (B) Time course of spatially averaged Cam4-ER ratio fluorescence in control R6 cells, and R6 cells expressing hBcl-2 or mBcl-2. Cells were incubated in Ca2+-free medium and stimulated with TG (1 μM) and ionomycin (iono) (1 μM) to completely deplete intracellular Ca2+ stores. The calibration procedure systematically performed at the end of each experiment is shown to illustrate the dynamic range (_R_max/_R_min) of the probe. (C) Basal [Ca2+]ER values (mean ± SEM) in control R6 cells versus hBcl-2 and mBcl-2 stable transfectants, as well as in control and Bcl-2-transfected HEK-293 cells. Measurements of [Ca2+]ER were made 3–4 days after transfection with Cam4-ER (R6 cells) or Cam4-ER plus Bcl-2 or control vector (HEK-293 cells). Results are derived from three independent experiments. *, P < 0.05; **, P < 0.0002.

Figure 4

Figure 4

Bcl-2 overexpression does not affect the pH of the ER. ER pH was measured by directly exciting at 480 nm the pH-dependent EYFP contained within the cameleon probe and measuring its fluorescence emission at 535 nm. HEK-293 cells cotransfected with Cam4-ER and hBcl-2 or control vector were used. (A) Time-course experiment showing the effect of TG and ionomycin (iono) on ER pH and the subsequent calibration with 130 mM KCl + 10 μM nigericin (nig) at pH 7.6 and 6.3. (B) pH values measured in the ER of control and hBcl-2 overexpressing cells, in basal condition and after store depletion with TG (1 μM) and ionomycin (1 μM). Data are mean ± SEM (n = 5) from two independent experiments in each condition.

Figure 5

Figure 5

Mitochondria and TG-insensitive Ca2+ pools in Bcl-2-expressing cells. (A) Typical [Ca2+]c traces of cells sequentially exposed to the mitochondrial inhibitor CCCP (1 μM) followed by ionomycin (iono) (100 nM). (B) Peak [Ca2+]c after addition of CCCP or solvent control (left bars) and after subsequent addition of ionomycin (right bars); mean ± SEM of three independent experiments. (C and E) Typical [Ca2+]c trace in cells sequentially exposed to TG (100 nM) for 0 min, CCCP (1 μM) for 5 min or 10 min, and ionomycin (100 nM) at 10 min or 15 min. (D and F) Peak [Ca2+]c after addition of CCCP (left bars) and after subsequent addition of ionomycin (right bars) to cells previously exposed to TG at t = 0. The time between TG and ionomycin addition was 5 min or 10 min for D and F, respectively.

Figure 6

Figure 6

Bcl-2 increases the permeability of ER-type Ca2+ stores. TG (100 nM) was added at time 0 min, and ionomycin (iono; 10 μM) was added either concomitantly with TG, or at later time points (1 min, 2 min, 3 min, 4, min, 5 min). (A and B) Typical experiments showing [Ca2+]c elevations in control and Bcl-2-transfected cells, respectively; four traces with ionomycin addition at different time points are superimposed to facilitate comparison. (C) Ionomycin-releasable Ca2+ (expressed as % of the initial peak [Ca2+]c) is plotted as a function of time after TG addition. (D) Time constant (τ) of Ca2+ store depletion, calculated for control and Bcl-2-transfected cells by fitting a mono-exponential decay function to the data of C. Results shown in C and D are mean ± SEM of four independent experiments.

Figure 7

Figure 7

Bcl-2 increases unstimulated Ca2+ influx and basal [Ca2+]c. (A and B) Cells were suspended in a Ca2+-free medium, and 1 mM Ca2+ was added to the medium after 5-min baseline recording. (A) Typical experiments showing [Ca2+]c elevations in control and Bcl-2-transfected cells without cellular stimulation. (B) Typical experiments showing [Ca2+]c elevations in control and Bcl-2-transfected cells after TG stimulation. (C) Basal [Ca2+]c of cells maintained in Ca2+-free medium or in Ca2+-containing medium; mean ± SEM of four independent experiments. (D) Statistical analysis of experiments as shown in A. (E) Statistical analysis of experiments as shown in B.

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