Single molecule microscopy of biomembranes (review) - PubMed (original) (raw)
Review
. 2000 Jan-Mar;17(1):17-29.
doi: 10.1080/096876800294452.
Affiliations
- PMID: 10824735
- DOI: 10.1080/096876800294452
Free article
Review
Single molecule microscopy of biomembranes (review)
G J Schütz et al. Mol Membr Biol. 2000 Jan-Mar.
Free article
Abstract
Recent advances in the development of new microscopy techniques with a sensitivity of a single molecule have gained access to essentially new types of information obtainable from imaging biomolecular samples. These methodologies are analysed here in terms of their applicability to the in vivo visualization of cellular processes on the molecular scale, in particular of processes in cell membranes. First examples of single molecule microscopy on cell membranes revealed new basic insight into the lateral organization of the plasma membrane, providing the captivating perspective of an ultrasensitive methodology as a general tool to study local processes and heterogeneities in living cells.
Similar articles
- Single molecule fluorescence and force microscopy.
Schütz GJ, Hinterdorfer P. Schütz GJ, et al. Exp Gerontol. 2002 Dec;37(12):1495-511. doi: 10.1016/s0531-5565(02)00124-9. Exp Gerontol. 2002. PMID: 12559419 Review. - Single molecule techniques for the study of membrane proteins.
García-Sáez AJ, Schwille P. García-Sáez AJ, et al. Appl Microbiol Biotechnol. 2007 Aug;76(2):257-66. doi: 10.1007/s00253-007-1007-8. Epub 2007 May 12. Appl Microbiol Biotechnol. 2007. PMID: 17497147 Review. - In situ single molecule imaging of cell membranes: linking basic nanotechniques to cell biology, immunology and medicine.
Pi J, Jin H, Yang F, Chen ZW, Cai J. Pi J, et al. Nanoscale. 2014 Nov 7;6(21):12229-49. doi: 10.1039/c4nr04195j. Nanoscale. 2014. PMID: 25227707 Review. - Nanophotonic approaches for nanoscale imaging and single-molecule detection at ultrahigh concentrations.
Mivelle M, Van Zanten TS, Manzo C, Garcia-Parajo MF. Mivelle M, et al. Microsc Res Tech. 2014 Jul;77(7):537-45. doi: 10.1002/jemt.22369. Epub 2014 Apr 7. Microsc Res Tech. 2014. PMID: 24710842 Review. - Ultra-sensitive fluorescence reader for bioanalysis.
Hesse J, Sonnleitner M, Schütz GJ. Hesse J, et al. Curr Pharm Biotechnol. 2004 Jun;5(3):309-19. doi: 10.2174/1389201043376850. Curr Pharm Biotechnol. 2004. PMID: 15180552 Review.
Cited by
- Limit of the Accuracy of Parameter Estimation for Moving Single Molecules Imaged by Fluorescence Microscopy.
Wong Y, Lin Z, Ober RJ. Wong Y, et al. IEEE Trans Signal Process. 2011;59(3):895-911. doi: 10.1109/TSP.2010.2098403. IEEE Trans Signal Process. 2011. PMID: 24665193 Free PMC article. - Whole cell patch clamp recording performed on a planar glass chip.
Fertig N, Blick RH, Behrends JC. Fertig N, et al. Biophys J. 2002 Jun;82(6):3056-62. doi: 10.1016/S0006-3495(02)75646-4. Biophys J. 2002. PMID: 12023228 Free PMC article. - Combined single-molecule force and fluorescence measurements for biology.
Wallace MI, Molloy JE, Trentham DR. Wallace MI, et al. J Biol. 2003;2(1):4. doi: 10.1186/1475-4924-2-4. Epub 2003 Apr 14. J Biol. 2003. PMID: 12733995 Free PMC article. Review. - Simultaneous optical and electrical recording of single gramicidin channels.
Borisenko V, Lougheed T, Hesse J, Füreder-Kitzmüller E, Fertig N, Behrends JC, Woolley GA, Schütz GJ. Borisenko V, et al. Biophys J. 2003 Jan;84(1):612-22. doi: 10.1016/S0006-3495(03)74881-4. Biophys J. 2003. PMID: 12524314 Free PMC article. - High intranuclear mobility and dynamic clustering of the splicing factor U1 snRNP observed by single particle tracking.
Kues T, Dickmanns A, Lührmann R, Peters R, Kubitscheck U. Kues T, et al. Proc Natl Acad Sci U S A. 2001 Oct 9;98(21):12021-6. doi: 10.1073/pnas.211250098. Epub 2001 Oct 2. Proc Natl Acad Sci U S A. 2001. PMID: 11593012 Free PMC article.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources