Analysis of expressed sequence tags indicates 35,000 human genes - PubMed (original) (raw)

Comparative Study

doi: 10.1038/76115.

Affiliations

• PMID: 10835644
• DOI: 10.1038/76115

Comparative Study

Analysis of expressed sequence tags indicates 35,000 human genes

B Ewing et al. Nat Genet. 2000 Jun.

Abstract

The number of protein-coding genes in an organism provides a useful first measure of its molecular complexity. Single-celled prokaryotes and eukaryotes typically have a few thousand genes; for example, Escherichia coli has 4,300 and Saccharomyces cerevisiae has 6,000. Evolution of multicellularity appears to have been accompanied by a several-fold increase in gene number, the invertebrates Caenorhabditis elegans and Drosophila melanogaster having 19,000 and 13,600 genes, respectively. Here we estimate the number of human genes by comparing a set of human expressed sequence tag (EST) contigs with human chromosome 22 and with a non-redundant set of mRNA sequences. The two comparisons give mutually consistent estimates of approximately 35,000 genes, substantially lower than most previous estimates. Evolution of the increased physiological complexity of vertebrates may therefore have depended more on the combinatorial diversification of regulatory networks or alternative splicing than on a substantial increase in gene number.

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Comment in

• The nature of the number.
  [No authors listed] [No authors listed] Nat Genet. 2000 Jun;25(2):127-8. doi: 10.1038/75946. Nat Genet. 2000. PMID: 10835616 No abstract available.
• How to count ... human genes.
  Aparicio SA. Aparicio SA. Nat Genet. 2000 Jun;25(2):129-30. doi: 10.1038/75949. Nat Genet. 2000. PMID: 10835617 No abstract available.

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