Drosophila Futsch/22C10 is a MAP1B-like protein required for dendritic and axonal development - PubMed (original) (raw)

Drosophila Futsch/22C10 is a MAP1B-like protein required for dendritic and axonal development

T Hummel et al. Neuron. 2000 May.

Free article

Abstract

Here we report the description of the Drosophila gene futsch, which encodes a protein recognized by the monoclonal antibody 22C10 that has been widely used to visualize neuronal morphology and axonal projections. The Futsch protein is 5327 amino acids in length. It localizes to the microtubule compartment of the cell and associates with microtubules in vitro. The N- and C-terminal domains of Futsch are homologous to the vertebrate MAP1B microtubule-associated protein. The central domain of the Futsch protein is highly repetitive and shows sequence similarity to neurofilament proteins of which no Drosophila homologs have been reported. Loss-of-function analyses demonstrate that during embryogenesis Futsch is necessary for dendritic and axonal growth. Gain-of-function analyses demonstrate a functional interaction of Futsch with other MAPs. In addition, we show that during development, futsch expression is negatively regulated in nonneuronal tissues.

PubMed Disclaimer

Similar articles

• The Drosophila microtubule associated protein Futsch is phosphorylated by Shaggy/Zeste-white 3 at an homologous GSK3beta phosphorylation site in MAP1B.
  Gögel S, Wakefield S, Tear G, Klämbt C, Gordon-Weeks PR. Gögel S, et al. Mol Cell Neurosci. 2006 Oct;33(2):188-99. doi: 10.1016/j.mcn.2006.07.004. Epub 2006 Sep 1. Mol Cell Neurosci. 2006. PMID: 16949836
• Drosophila Futsch regulates synaptic microtubule organization and is necessary for synaptic growth.
  Roos J, Hummel T, Ng N, Klämbt C, Davis GW. Roos J, et al. Neuron. 2000 May;26(2):371-82. doi: 10.1016/s0896-6273(00)81170-8. Neuron. 2000. PMID: 10839356
• Disruption of the MAP1B-related protein FUTSCH leads to changes in the neuronal cytoskeleton, axonal transport defects, and progressive neurodegeneration in Drosophila.
  Bettencourt da Cruz A, Schwärzel M, Schulze S, Niyyati M, Heisenberg M, Kretzschmar D. Bettencourt da Cruz A, et al. Mol Biol Cell. 2005 May;16(5):2433-42. doi: 10.1091/mbc.e04-11-1004. Epub 2005 Mar 16. Mol Biol Cell. 2005. PMID: 15772149 Free PMC article.
• Highwire, rpm-1, and futsch: balancing synaptic growth and stability.
  Chang Q, Balice-Gordon RJ. Chang Q, et al. Neuron. 2000 May;26(2):287-90. doi: 10.1016/s0896-6273(00)81161-7. Neuron. 2000. PMID: 10839347 Review. No abstract available.
• MAP1B expression and microtubule stability in growing and regenerating axons.
  Gordon-Weeks PR, Fischer I. Gordon-Weeks PR, et al. Microsc Res Tech. 2000 Jan 15;48(2):63-74. doi: 10.1002/(SICI)1097-0029(20000115)48:2<63::AID-JEMT2>3.0.CO;2-1. Microsc Res Tech. 2000. PMID: 10649507 Review.

Cited by

• Dynein light chain 1 (LC8) association enhances microtubule stability and promotes microtubule bundling.
  Asthana J, Kuchibhatla A, Jana SC, Ray K, Panda D. Asthana J, et al. J Biol Chem. 2012 Nov 23;287(48):40793-805. doi: 10.1074/jbc.M112.394353. Epub 2012 Oct 4. J Biol Chem. 2012. PMID: 23038268 Free PMC article.
• The Presynaptic Microtubule Cytoskeleton in Physiological and Pathological Conditions: Lessons from Drosophila Fragile X Syndrome and Hereditary Spastic Paraplegias.
  Bodaleo FJ, Gonzalez-Billault C. Bodaleo FJ, et al. Front Mol Neurosci. 2016 Jul 25;9:60. doi: 10.3389/fnmol.2016.00060. eCollection 2016. Front Mol Neurosci. 2016. PMID: 27504085 Free PMC article. Review.
• Functional conservation of human Spastin in a Drosophila model of autosomal dominant-hereditary spastic paraplegia.
  Du F, Ozdowski EF, Kotowski IK, Marchuk DA, Sherwood NT. Du F, et al. Hum Mol Genet. 2010 May 15;19(10):1883-96. doi: 10.1093/hmg/ddq064. Epub 2010 Feb 13. Hum Mol Genet. 2010. PMID: 20154342 Free PMC article.
• Novel isoforms of Dlg are fundamental for neuronal development in Drosophila.
  Mendoza C, Olguín P, Lafferte G, Thomas U, Ebitsch S, Gundelfinger ED, Kukuljan M, Sierralta J. Mendoza C, et al. J Neurosci. 2003 Mar 15;23(6):2093-101. doi: 10.1523/JNEUROSCI.23-06-02093.2003. J Neurosci. 2003. PMID: 12657668 Free PMC article.
• MAP1B is required for axon guidance and Is involved in the development of the central and peripheral nervous system.
  Meixner A, Haverkamp S, Wässle H, Führer S, Thalhammer J, Kropf N, Bittner RE, Lassmann H, Wiche G, Propst F. Meixner A, et al. J Cell Biol. 2000 Dec 11;151(6):1169-78. doi: 10.1083/jcb.151.6.1169. J Cell Biol. 2000. PMID: 11121433 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Molecular Biology Databases

  • BioCyc
  • FlyBase
  • Gene Ontology
  • GlyGen glycoinformatics resource

• Miscellaneous

  • SciCrunch