Gut instincts: thoughts on intestinal epithelial stem cells - PubMed (original) (raw)

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Gut instincts: thoughts on intestinal epithelial stem cells

C Booth et al. J Clin Invest. 2000 Jun.

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Figures

Figure 1

Figure 1

A small intestinal crypt (stained with hematoxylin and eosin) with autoradiographic silver grains overlying a single nucleus in the stem-cell region. This label-retaining cell was obtained by administering tritiated thymidine every 6 hours for 48 hours after irradiation with 8 Gy. The sample was taken 8 days later.

Figure 2

Figure 2

(a and b) Examples of “spontaneous” crypt fission (bifurcation) in normal adult mouse small intestine. (c) Multiple fission after irradiation with 12 Gy. A new crypt can be seen developing from the mid-crypt region as well as from the crypt base. Each neocrypt contains Paneth’s cells. (d) Similar bifurcation from a higher crypt-cell position can be seen in the colon after irradiation (10 Gy).

Figure 3

Figure 3

A human small intestinal crypt immunostained for telomerase. Labeling can be seen toward the crypt base, indicating telomere regeneration in the stem cell region.

Figure 4

Figure 4

(a and b) Four-week-old grafts of colonic epithelium derived from isolated crypts. Invaginations similar to those observed during normal crypt development can be seen, with differentiated goblet cells and secreting mucins at the apical pole, and proliferating cells (labeled with tritiated thymidine) at the basal pole. (c) At later times some of these grafts develop a structure more typical of adult intestinal crypts. Adapted from ref. .

References

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