Neuroanatomy of cells expressing clock genes in Drosophila: transgenic manipulation of the period and timeless genes to mark the perikarya of circadian pacemaker neurons and their projections - PubMed (original) (raw)
Neuroanatomy of cells expressing clock genes in Drosophila: transgenic manipulation of the period and timeless genes to mark the perikarya of circadian pacemaker neurons and their projections
M Kaneko et al. J Comp Neurol. 2000.
Abstract
Subsets of brain neurons expressing the clock genes period (per) and timeless (tim) are involved in the generation of circadian behavioral rhythms. However, current knowledge of projection patterns of these neurons is limited to those immunoreactive to an antibody against a crustacean neuropeptide. The GAL4-expression system was utilized to visualize neuronal processes from all per and tim-expressing neurons in the central nervous system. Each of two types of GAL4-driver fusion genes, per-gal4 or tim-gal4, was combined in transgenic flies with marker genes-lacZ, and sequences encoding green fluorescent protein or TAU protein-under the control of the GAL4-responsive element UAS. This allowed visualization of the cytoplasm of GAL4-expressing cells. Thus, neurites of clock neurons in the adult brain as well as those of larvae and pupae were revealed. Among the anatomical patterns revealed by per-gal4- or tim-gal4-driven marker expression were a previously unknown, dorsally located neuronal cluster, along with the projections of these cells and of other dorsal neurons characterized in earlier studies only by the location of their perikarya. The similarity of projections from PER- or TIM-containing neurons during development to those in the adult implies that these features of mature clock neurons are established by the larval stages. Neurons that have never been identified as PER- or TIM-immunoreactive were also visualized in this assay system, indicating promoter activity of the clock genes in these cells and suggesting that their products cannot accumulate to detectable levels in certain neurons.
Copyright 2000 Wiley-Liss, Inc.
Similar articles
- Disruption of synaptic transmission or clock-gene-product oscillations in circadian pacemaker cells of Drosophila cause abnormal behavioral rhythms.
Kaneko M, Park JH, Cheng Y, Hardin PE, Hall JC. Kaneko M, et al. J Neurobiol. 2000 Jun 5;43(3):207-33. doi: 10.1002/(sici)1097-4695(20000605)43:3<207::aid-neu1>3.0.co;2-0. J Neurobiol. 2000. PMID: 10842235 - Sequential nuclear accumulation of the clock proteins period and timeless in the pacemaker neurons of Drosophila melanogaster.
Shafer OT, Rosbash M, Truman JW. Shafer OT, et al. J Neurosci. 2002 Jul 15;22(14):5946-54. doi: 10.1523/JNEUROSCI.22-14-05946.2002. J Neurosci. 2002. PMID: 12122057 Free PMC article. - Circadian clock genes in Drosophila: recent developments.
Subramanian P, Balamurugan E, Suthakar G. Subramanian P, et al. Indian J Exp Biol. 2003 Aug;41(8):797-804. Indian J Exp Biol. 2003. PMID: 15248475 Review. - Partners in time. Circadian rhythms.
Van Gelder RN, Krasnow MA. Van Gelder RN, et al. Curr Biol. 1996 Mar 1;6(3):244-6. doi: 10.1016/s0960-9822(02)00468-2. Curr Biol. 1996. PMID: 8805241 Review.
Cited by
- Reciprocal cholinergic and GABAergic modulation of the small ventrolateral pacemaker neurons of Drosophila's circadian clock neuron network.
Lelito KR, Shafer OT. Lelito KR, et al. J Neurophysiol. 2012 Apr;107(8):2096-108. doi: 10.1152/jn.00931.2011. Epub 2012 Jan 25. J Neurophysiol. 2012. PMID: 22279191 Free PMC article. - Development-based compartmentalization of the Drosophila central brain.
Pereanu W, Kumar A, Jennett A, Reichert H, Hartenstein V. Pereanu W, et al. J Comp Neurol. 2010 Aug 1;518(15):2996-3023. doi: 10.1002/cne.22376. J Comp Neurol. 2010. PMID: 20533357 Free PMC article. - Genome-Wide Association Study of Circadian Behavior in Drosophila melanogaster.
Harbison ST, Kumar S, Huang W, McCoy LJ, Smith KR, Mackay TFC. Harbison ST, et al. Behav Genet. 2019 Jan;49(1):60-82. doi: 10.1007/s10519-018-9932-0. Epub 2018 Oct 19. Behav Genet. 2019. PMID: 30341464 Free PMC article. - Aging and the clock: Perspective from flies to humans.
De Nobrega AK, Lyons LC. De Nobrega AK, et al. Eur J Neurosci. 2020 Jan;51(1):454-481. doi: 10.1111/ejn.14176. Epub 2018 Oct 30. Eur J Neurosci. 2020. PMID: 30269400 Free PMC article. Review. - Surprising gene expression patterns within and between PDF-containing circadian neurons in Drosophila.
Kula-Eversole E, Nagoshi E, Shang Y, Rodriguez J, Allada R, Rosbash M. Kula-Eversole E, et al. Proc Natl Acad Sci U S A. 2010 Jul 27;107(30):13497-502. doi: 10.1073/pnas.1002081107. Epub 2010 Jul 12. Proc Natl Acad Sci U S A. 2010. PMID: 20624977 Free PMC article.