Comparison of conserved structural and regulatory domains within divergent 16S rRNA-23S rRNA spacer sequences of cyanobacteria - PubMed (original) (raw)
Comparative Study
. 2000 Jun:146 ( Pt 6):1275-1286.
doi: 10.1099/00221287-146-6-1275.
Affiliations
- PMID: 10846207
- DOI: 10.1099/00221287-146-6-1275
Free article
Comparative Study
Comparison of conserved structural and regulatory domains within divergent 16S rRNA-23S rRNA spacer sequences of cyanobacteria
Isabelle Iteman et al. Microbiology (Reading). 2000 Jun.
Free article
Abstract
PCR amplification of the internal transcribed spacer (ITS) between the 16S rRNA and 23S rRNA genes of the cyanobacterium NOSTOC: PCC 7120 gave three products. Two represented true ITS regions of different sizes, while the third was a heteroduplex. The longer spacer (ITS-L) contained 512 nucleotides and carried tRNA(Ile) and tRNA(Ala) genes, separated by a large stem-loop structure (V2) composed of short tandemly repeated repetitive sequences. Both tRNA genes, and the 5' half of the intervening stem, were absent from the shorter spacer (ITS-S), of length 283 nucleotides, which was otherwise almost completely identical to ITS-L. The two spacer regions of NOSTOC: PCC 7120 were aligned to published ITS sequences of cyanobacteria, the cyanelle of Cyanophora paradoxa and Escherichia coli. Although the ITS regions of cyanobacteria vary in length from 283 to 545 nucleotides and contain either both tRNA(Ile) and tRNA(Ala) genes, only the tRNA(Ile) gene, or neither, there is no correlation between ITS size and coding capacity for tRNAs. Putative secondary structures were determined for the deduced transcripts of the rrn operons of several cyanobacteria and were compared to that of E. coli. Highly conserved motifs important for folding and for maturation of the rRNA transcripts were identified, and regions homologous to bacterial antiterminators (box B-box A) were located. The conserved and variable regions of the cyanobacterial ITS are potential targets of PCR primers and oligonucleotide probes for detection and identification of cyanobacteria at different taxonomic levels.
Similar articles
- Sequence diversity in the 16S-23S intergenic spacer region (ISR) of the rRNA operons in representatives of the Escherichia coli ECOR collection.
Antón AI, Martínez-Murcia AJ, Rodríguez-Valera F. Antón AI, et al. J Mol Evol. 1998 Jul;47(1):62-72. doi: 10.1007/pl00006363. J Mol Evol. 1998. PMID: 9664697 - Molecular characterization of DNA encoding 23S rRNA and 16S-23S rRNA intergenic spacer regions of Aeromonas hydrophila.
East AK, Collins MD. East AK, et al. FEMS Microbiol Lett. 1993 Jan 15;106(2):129-33. doi: 10.1111/j.1574-6968.1993.tb05947.x. FEMS Microbiol Lett. 1993. PMID: 7681020 - tRNA intergenic spacers reveal polymorphisms diagnostic for Xanthomonas albilineans.
Honeycutt RJ, Sobral BW, McClelland M. Honeycutt RJ, et al. Microbiology (Reading). 1995 Dec;141 ( Pt 12):3229-39. doi: 10.1099/13500872-141-12-3229. Microbiology (Reading). 1995. PMID: 8574414 - Lessons from an evolving rRNA: 16S and 23S rRNA structures from a comparative perspective.
Gutell RR, Larsen N, Woese CR. Gutell RR, et al. Microbiol Rev. 1994 Mar;58(1):10-26. doi: 10.1128/mr.58.1.10-26.1994. Microbiol Rev. 1994. PMID: 8177168 Free PMC article. Review. - The molecular evolution and DNA profiling of toxic cyanobacteria.
Neilan BA. Neilan BA. Curr Issues Mol Biol. 2002 Jan;4(1):1-11. Curr Issues Mol Biol. 2002. PMID: 11838942 Review.
Cited by
- Development of Leptolyngbya sp. BL0902 into a model organism for synthetic biological research in filamentous cyanobacteria.
Gao H, Wang Y, Huang Z, Yu F, Hu X, Ning D, Xu X. Gao H, et al. Front Microbiol. 2024 Jul 22;15:1409771. doi: 10.3389/fmicb.2024.1409771. eCollection 2024. Front Microbiol. 2024. PMID: 39104590 Free PMC article. - A multiplex Taqman PCR assay for MRSA detection from whole blood.
Duraiswamy S, Agarwalla S, Lok KS, Tse YY, Wu R, Wang Z. Duraiswamy S, et al. PLoS One. 2023 Nov 27;18(11):e0294782. doi: 10.1371/journal.pone.0294782. eCollection 2023. PLoS One. 2023. PMID: 38011181 Free PMC article. - Polyphasic characterization of a novel hot-spring cyanobacterium Thermocoleostomius sinensis gen et sp. nov. and genomic insights into its carbon concentration mechanism.
Jiang Y, Tang J, Liu X, Daroch M. Jiang Y, et al. Front Microbiol. 2023 Jul 26;14:1176500. doi: 10.3389/fmicb.2023.1176500. eCollection 2023. Front Microbiol. 2023. PMID: 37564287 Free PMC article. - Characterization of a novel thermophilic cyanobacterium within Trichocoleusaceae, Trichothermofontia sichuanensis gen. et sp. nov., and its CO2-concentrating mechanism.
Tang J, Zhou H, Jiang Y, Yao D, Waleron KF, Du LM, Daroch M. Tang J, et al. Front Microbiol. 2023 Apr 27;14:1111809. doi: 10.3389/fmicb.2023.1111809. eCollection 2023. Front Microbiol. 2023. PMID: 37180226 Free PMC article. - High-resolution Spatiotemporal Dynamics of Harmful Algae in the Indian River Lagoon (Florida)-A Case Study of Aureoumbra lagunensis, Pyrodinium bahamense, and _Pseudo_-nitzschia.
Lopez CB, Tilney CL, Muhlbach E, Bouchard JN, Villac MC, Henschen KL, Markley LR, Abbe SK, Shankar S, Shea C, Flewelling L, Garrett M, Badylak S, Phlips EJ, Hall LM, Lasi MA, Parks A, Paperno R, Adams DH, Edwards DD, Schneider JE, Wald KB, Biddle AR, Landers SL, Hubbard KA. Lopez CB, et al. Front Mar Sci. 2021;8:769877. doi: 10.3389/fmars.2021.769877. Epub 2021 Nov 24. Front Mar Sci. 2021. PMID: 37065006 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources