An interlaboratory comparison of physiological and genetic properties of four Saccharomyces cerevisiae strains - PubMed (original) (raw)

. 2000 Jun 1;26(9-10):706-714.

doi: 10.1016/s0141-0229(00)00162-9.

J Bauer, L Brambilla, P Duboc, JM Francois, C Gancedo, ML Giuseppin, JJ Heijnen, M Hoare, HC Lange, EA Madden, P Niederberger, J Nielsen, JL Parrou, T Petit, D Porro, M Reuss, van Riel N, M Rizzi, HY Steensma, CT Verrips, J Vindeløv, JT Pronk

Affiliations

An interlaboratory comparison of physiological and genetic properties of four Saccharomyces cerevisiae strains

van Dijken JP et al. Enzyme Microb Technol. 2000.

Abstract

To select a Saccharomyces cerevisiae reference strain amenable to experimental techniques used in (molecular) genetic, physiological and biochemical engineering research, a variety of properties were studied in four diploid, prototrophic laboratory strains. The following parameters were investigated: 1) maximum specific growth rate in shake-flask cultures; 2) biomass yields on glucose during growth on defined media in batch cultures and steady-state chemostat cultures under controlled conditions with respect to pH and dissolved oxygen concentration; 3) the critical specific growth rate above which aerobic fermentation becomes apparent in glucose-limited accelerostat cultures; 4) sporulation and mating efficiency; and 5) transformation efficiency via the lithium-acetate, bicine, and electroporation methods. On the basis of physiological as well as genetic properties, strains from the CEN.PK family were selected as a platform for cell-factory research on the stoichiometry and kinetics of growth and product formation.

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