DNA fingerprinting of a national sample of Mycobacterium tuberculosis isolates, Botswana, 1995-1996 - PubMed (original) (raw)
Affiliations
- PMID: 10864192
DNA fingerprinting of a national sample of Mycobacterium tuberculosis isolates, Botswana, 1995-1996
S Lockman et al. Int J Tuberc Lung Dis. 2000 Jun.
Abstract
DNA fingerprinting may be useful to elucidate tuberculosis (TB) transmission in community settings, but its utility is limited if only few fingerprint patterns are observed or band numbers are low. We performed DNA fingerprinting on a national, population-based sample of Mycobacterium tuberculosis isolates from Botswana. During 1995-1996, a random sample of 213 isolates, representing 5% of all smear-positive TB cases, underwent DNA fingerprinting using restriction fragment length polymorphism (RFLP) IS6110 analysis. Eighty-two (38%) of the 213 isolates belonged to one of 18 clusters, with 2-9 isolates/cluster. The median number of bands was 10 (range 1-19); 183 (86%) had six or more bands. Sixty-three (49%) of 128 patients tested were infected with the human immunodeficiency virus (HIV). The degree of RFLP pattern heterogeneity and high band number support the feasibility of a prospective DNA fingerprinting study in Botswana.
Similar articles
- Molecular and conventional epidemiology of Mycobacterium tuberculosis in Botswana: a population-based prospective study of 301 pulmonary tuberculosis patients.
Lockman S, Sheppard JD, Braden CR, Mwasekaga MJ, Woodley CL, Kenyon TA, Binkin NJ, Steinman M, Montsho F, Kesupile-Reed M, Hirschfeldt C, Notha M, Moeti T, Tappero JW. Lockman S, et al. J Clin Microbiol. 2001 Mar;39(3):1042-7. doi: 10.1128/JCM.39.3.1042-1047.2001. J Clin Microbiol. 2001. PMID: 11230425 Free PMC article. - Determination of drug susceptibility and DNA fingerprint patterns of clinical isolates of Mycobacterium tuberculosis from Kampala, Uganda.
Joloba ML, Whalen CC, Cave DM, Eisenach KD, Johnson JL, Okwera A, Morrissey A, Bajaksouzian S, Feagin J, Mugerwa R, Ellner J, Jacobs MR. Joloba ML, et al. East Afr Med J. 2000 Feb;77(2):111-5. East Afr Med J. 2000. PMID: 10774085 - A prospective evaluation of the clinical value of nation-wide DNA fingerprinting of tuberculosis isolates in Denmark.
Bauer J, Kok-Jensen A, Faurschou P, Thuesen J, Taudorf E, Andersen AB. Bauer J, et al. Int J Tuberc Lung Dis. 2000 Apr;4(4):295-9. Int J Tuberc Lung Dis. 2000. PMID: 10777076 - [New era in molecular epidemiology of tuberculosis in Japan].
Takashima T, Iwamoto T. Takashima T, et al. Kekkaku. 2006 Nov;81(11):693-707. Kekkaku. 2006. PMID: 17154049 Review. Japanese. - How close is close enough? Exploring matching criteria in the estimation of recent transmission of tuberculosis.
Benedetti A, Menzies D, Behr MA, Schwartzman K, Jin Y. Benedetti A, et al. Am J Epidemiol. 2010 Aug 1;172(3):318-26. doi: 10.1093/aje/kwq124. Epub 2010 Jun 24. Am J Epidemiol. 2010. PMID: 20576754 Review.
Cited by
- Genetic diversity of Mycobacterium tuberculosis strains circulating in Botswana.
Mogashoa T, Melamu P, Ley SD, Streicher EM, Iketleng T, Kelentse N, Mupfumi L, Mokomane M, Kgwaadira B, Novitsky V, Kasvosve I, Moyo S, Warren RM, Gaseitsiwe S. Mogashoa T, et al. PLoS One. 2019 May 7;14(5):e0216306. doi: 10.1371/journal.pone.0216306. eCollection 2019. PLoS One. 2019. PMID: 31063472 Free PMC article. Clinical Trial. - Bayesian estimation of mixture models with prespecified elements to compare drug resistance in treatment-naïve and experienced tuberculosis cases.
Izu A, Cohen T, Degruttola V. Izu A, et al. PLoS Comput Biol. 2013;9(3):e1002973. doi: 10.1371/journal.pcbi.1002973. Epub 2013 Mar 21. PLoS Comput Biol. 2013. PMID: 23555210 Free PMC article. - Diversity of Mycobacterium tuberculosis genotypes circulating in Ndola, Zambia.
Mulenga C, Shamputa IC, Mwakazanga D, Kapata N, Portaels F, Rigouts L. Mulenga C, et al. BMC Infect Dis. 2010 Jun 17;10:177. doi: 10.1186/1471-2334-10-177. BMC Infect Dis. 2010. PMID: 20565802 Free PMC article. - Molecular epidemiology and drug resistance of Mycobacterium tuberculosis isolates from Ethiopian pulmonary tuberculosis patients with and without human immunodeficiency virus infection.
Bruchfeld J, Aderaye G, Palme IB, Bjorvatn B, Ghebremichael S, Hoffner S, Lindquist L. Bruchfeld J, et al. J Clin Microbiol. 2002 May;40(5):1636-43. doi: 10.1128/JCM.40.5.1636-1643.2002. J Clin Microbiol. 2002. PMID: 11980933 Free PMC article. - Molecular and conventional epidemiology of Mycobacterium tuberculosis in Botswana: a population-based prospective study of 301 pulmonary tuberculosis patients.
Lockman S, Sheppard JD, Braden CR, Mwasekaga MJ, Woodley CL, Kenyon TA, Binkin NJ, Steinman M, Montsho F, Kesupile-Reed M, Hirschfeldt C, Notha M, Moeti T, Tappero JW. Lockman S, et al. J Clin Microbiol. 2001 Mar;39(3):1042-7. doi: 10.1128/JCM.39.3.1042-1047.2001. J Clin Microbiol. 2001. PMID: 11230425 Free PMC article.