Pulsed-field gel electrophoresis of degradation-sensitive DNAs from Clostridium difficile PCR ribotype 1 strains - PubMed (original) (raw)

Pulsed-field gel electrophoresis of degradation-sensitive DNAs from Clostridium difficile PCR ribotype 1 strains

J E Corkill et al. J Clin Microbiol. 2000 Jul.

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Figures

FIG. 1

PFGE patterns obtained with _Sma_I-digested DNA from C. difficile isolates of known PCR ribotype. Electrophoresis was performed in the absence (a) and in the presence (b) of 50 μM thiourea. Lanes 1 to 5, five degradation-sensitive PCR ribotype 1 isolates (from the United States and Newcastle, Wrexham, Tooting, and Truro, United Kingdom, respectively); lane 6, ribotype 2 isolate (Wrexham); lane 7, ribotype 10 isolate (Manchester, United Kingdom); lane 8, ribotype 15 isolate (Carshalton, United Kingdom); lane 9, ribotype 23 isolate (Jersey, United Kingdom); lane 10, λ DNA standard (48.5-kbp concatemer).

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