dpy-18 encodes an alpha-subunit of prolyl-4-hydroxylase in caenorhabditis elegans - PubMed (original) (raw)

dpy-18 encodes an alpha-subunit of prolyl-4-hydroxylase in caenorhabditis elegans

K L Hill et al. Genetics. 2000 Jul.

Abstract

Collagen is an extracellular matrix (ECM) component encoded by a large multigene family in multicellular animals. Procollagen is post-translationally modified by prolyl-4-hydroxylase (EC 1.14.11.2) before secretion and participation in ECM formation. Therefore, collagen processing and regulation can be studied by examining this required interaction of prolyl-4-hydroxylase with procollagen. High-resolution polymorphism mapping was used to place the Caenorhabditis elegans dpy-18 gene on the physical map, and we show that it encodes a prolyl-4-hydroxylase alpha catalytic subunit. The Dpy phenotype of dpy-18(e364) amber mutants is more severe when this mutation is in trans to the noncomplementing deficiency tDf7, while the dpy-18(e499) deletion mutant exhibits the same phenotype as dpy-18(e499)/tDf7. Furthermore, dpy-18 RNA interference (RNAi) in wild-type worms results in Dpy progeny, while dpy-18 (RNAi) in dpy-18(e499) mutants does not alter the Dpy phenotype of their progeny. These observations suggest that the dpy-18 null phenotype is Dpy. A dpy-18::gfp promoter fusion construct is expressed throughout the hypodermis within the cells that abundantly produce the cuticle collagens, as well as in certain head and posterior neurons. While prolyl-4-hydroxylase has been studied extensively by biochemical techniques, this is the first report of a mutationally defined prolyl-4-hydroxylase in any animal.

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References

1. 1. Genetics. 1981 Feb;97(2):307-25 - PubMed
2. 1. Cell. 1998 Apr 3;93(1):71-9 - PubMed
3. 1. Genetics. 1999 Oct;153(2):743-52 - PubMed
4. 1. Mol Cell Biol. 1985 Feb;5(2):363-72 - PubMed
5. 1. Genetics. 1983 Jan;103(1):43-64 - PubMed

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