Spreading and motility of human glioblastoma cells on sheets of silicone rubber depend on substratum compliance - PubMed (original) (raw)

Spreading and motility of human glioblastoma cells on sheets of silicone rubber depend on substratum compliance

T W Thomas et al. Med Biol Eng Comput. 2000 May.

Abstract

Although there is a substantial quantity of experimental data examining the effects of adhesion on the morphology and migration of tissue cells, little attention has been focused on how changes in substratum mechanical properties affect these cellular behaviours. To determine whether the ability of a substratum mechanically to support traction influences cell morphology and motility, measurements are taken of the spreading, the fraction of a population with pseudopodia, the number of pseudopodia and the translocation of human SNB-19 glioblastoma cells cultured on films of poly(methylphenyl)siloxane possessing a range of mechanical compliances. Cells cultured on these films generate deformations (i.e. 'wrinkles') that are used as a basis to estimate effective substratum compliances. The average projected cell area decreases by over 60%, with a two-orders-of-magnitude increase in compliance. Time-lapse videomicroscopy reveals that cell migration also decreases with increasing compliance: the average cell speed decreases from approximately 8 microns h-1 on the most rigid substrata to 1.2 microns h-1 on the most compliant substrata examined. Changes in compliance do not alter mean directional persistence time. These results are interpreted in terms of the predictions of mathematical models for the effects of substratum compliance on motility.

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