Flow cytometric analysis of the Vbeta repertoire in healthy controls - PubMed (original) (raw)
Flow cytometric analysis of the Vbeta repertoire in healthy controls
R van den Beemd et al. Cytometry. 2000.
Free article
Abstract
Background: Analysis of the T-cell receptor (TCR)-Vbeta repertoire has been used for studying selective T-cell responses in autoimmune disease, alloreactivity in transplantation, and protective immunity against microbial and tumor antigens. For the interpretation of these studies, we need information about the Vbeta repertoire usage in healthy individuals.
Methods: We analyzed blood T-lymphocyte (sub)populations of 36 healthy controls (age range: from neonates to 86 years) with a carefully selected most complete panel of 22 Vbeta monoclonal antibodies, which together recognized 70-75% of all blood TCRalphabeta(+) T lymphocytes. Subsequently, we developed a six-tube test kit with selected Vbeta antibody combinations for easy and rapid detection of single ("clonal") Vbeta domain usage in large T-cell expansions.
Results: The mean values of the Vbeta repertoire usage were stable during aging in blood TCRalphabeta(+) T lymphocytes as well as in the CD4(+) and CD8(+) T-cell subsets, although the standard deviations increased in the elderly. The increased standard deviations were caused by the occurrence of oligoclonal T-cell expansions in the elderly, mainly consisting of CD8(+) T lymphocytes. The 15 detected T-cell expansions did not reach 40% of total TCRalphabeta(+) T lymphocytes and represented less than 0.4 x 10(9) cells per liter in our study. Vbeta usage of the CD4(+) and CD8(+) subsets was comparable for most tested Vbeta domains, but significant differences (P < 0.01) between the two subsets were found for Vbeta2, Vbeta5.1, Vbeta6.7, Vbeta9.1, and Vbeta22 (higher in CD4(+)), as well as for Vbeta1, Vbeta7.1, Vbeta14, and Vbeta23 (higher in CD8(+)). Finally, single Vbeta domain expression in large T-cell expansions can indeed be detected by the six-tube test kit.
Conclusions: The results of our study can now be used as reference values in studies on distortions of the Vbeta repertoire in disease states. The six-tube test kit can be used for detection of single Vbeta domain expression in large T-cell expansions (>2.0 x 10(9)/l), which are clinically suspicious of T-cell leukemia.
Copyright 2000 Wiley-Liss, Inc.
Comment in
- T-cell receptor analysis by flow cytometry.
McCoy P. McCoy P. Cytometry. 2001 Aug 1;44(4):369. doi: 10.1002/1097-0320(20010801)44:4<369::aid-cyto1129>3.0.co;2-d. Cytometry. 2001. PMID: 11500854 No abstract available.
Similar articles
- The Value of Flow Cytometry Clonality in Large Granular Lymphocyte Leukemia.
Giudice V, D'Addona M, Montuori N, Selleri C. Giudice V, et al. Cancers (Basel). 2021 Sep 8;13(18):4513. doi: 10.3390/cancers13184513. Cancers (Basel). 2021. PMID: 34572739 Free PMC article. Review. - Flow cytometric analysis of TCR Vβ repertoire in patients with 22q11.2 deletion syndrome.
McLean-Tooke A, Barge D, Spickett GP, Gennery AR. McLean-Tooke A, et al. Scand J Immunol. 2011 Jun;73(6):577-85. doi: 10.1111/j.1365-3083.2011.02527.x. Scand J Immunol. 2011. PMID: 21323691 - Immunophenotypic analysis of the TCR-Vbeta repertoire in 98 persistent expansions of CD3(+)/TCR-alphabeta(+) large granular lymphocytes: utility in assessing clonality and insights into the pathogenesis of the disease.
Lima M, Almeida J, Santos AH, dos Anjos Teixeira M, Alguero MC, Queirós ML, Balanzategui A, Justiça B, Gonzalez M, San Miguel JF, Orfão A. Lima M, et al. Am J Pathol. 2001 Nov;159(5):1861-8. doi: 10.1016/s0002-9440(10)63032-5. Am J Pathol. 2001. PMID: 11696446 Free PMC article. - T-cell receptor repertoire in healthy Sardinian subjects.
Bonfigli S, Doro MG, Fozza C, Derudas D, Dore F, Longinotti M. Bonfigli S, et al. Hum Immunol. 2003 Jul;64(7):689-95. doi: 10.1016/s0198-8859(03)00086-7. Hum Immunol. 2003. PMID: 12826371
Cited by
- Generation and optimization of off-the-shelf immunotherapeutics targeting TCR-Vβ2+ T cell malignancy.
Ren J, Liao X, Lewis JM, Chang J, Qu R, Carlson KR, Foss F, Girardi M. Ren J, et al. Nat Commun. 2024 Jan 15;15(1):519. doi: 10.1038/s41467-024-44786-2. Nat Commun. 2024. PMID: 38225288 Free PMC article. - Patient-Specific Targeting of the T-Cell Receptor Variable Region as a Therapeutic Strategy in Clonal T-Cell Diseases.
Lucero OM, Lee JA, Bowman J, Johnson K, Sapparapu G, Thomas JK, Fan G, Chang BH, Thiel-Klare K, Eide CA, Okada C, Palazzolo M, Lind E, Kosaka Y, Druker BJ, Lydon N, Bowers PM. Lucero OM, et al. Clin Cancer Res. 2023 Oct 13;29(20):4230-4241. doi: 10.1158/1078-0432.CCR-22-0906. Clin Cancer Res. 2023. PMID: 37199721 Free PMC article. - Flow cytometry for the assessment of blood tumour burden in cutaneous T-cell lymphoma: towards a standardized approach.
Vermeer MH, Moins-Teisserenc H, Bagot M, Quaglino P, Whittaker S. Vermeer MH, et al. Br J Dermatol. 2022 Jul;187(1):21-28. doi: 10.1111/bjd.21053. Epub 2022 May 3. Br J Dermatol. 2022. PMID: 35157307 Free PMC article. Review. - The Value of Flow Cytometry Clonality in Large Granular Lymphocyte Leukemia.
Giudice V, D'Addona M, Montuori N, Selleri C. Giudice V, et al. Cancers (Basel). 2021 Sep 8;13(18):4513. doi: 10.3390/cancers13184513. Cancers (Basel). 2021. PMID: 34572739 Free PMC article. Review. - T cell regeneration after immunological injury.
Velardi E, Tsai JJ, van den Brink MRM. Velardi E, et al. Nat Rev Immunol. 2021 May;21(5):277-291. doi: 10.1038/s41577-020-00457-z. Epub 2020 Oct 23. Nat Rev Immunol. 2021. PMID: 33097917 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials