Changes in bacterial community structure in the colon of pigs fed different experimental diets and after infection with Brachyspira hyodysenteriae - PubMed (original) (raw)

Changes in bacterial community structure in the colon of pigs fed different experimental diets and after infection with Brachyspira hyodysenteriae

T D Leser et al. Appl Environ Microbiol. 2000 Aug.

Abstract

Bacterial communities in the large intestines of pigs were compared using terminal restriction fragment length polymorphism (T-RFLP) analysis targeting the 16S ribosomal DNA. The pigs were fed different experimental diets based on either modified standard feed or cooked rice supplemented with dietary fibers. After feeding of the animals with the experimental diets for 2 weeks, differences in the bacterial community structure in the spiral colon were detected in the form of different profiles of terminal restriction fragments (T-RFs). Some of the T-RFs were universally distributed, i.e., they were found in all samples, while others varied in distribution and were related to specific diets. The reproducibility of the T-RFLP profiles between individual animals within the diet groups was high. In the control group, the profiles remained unchanged throughout the experiment and were similar between two independent but identical experiments. When the animals were experimentally infected with Brachyspira hyodysenteriae, causing swine dysentery, many of the T-RFs fluctuated, suggesting a destabilization of the microbial community.

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Figures

FIG. 1

FIG. 1

T-RFLP profiles from the colon lumenal contents of two pigs in the control group from experiment 1. K-31 was sampled after the animals had been fed the standard diet for 2 weeks; K-19 was sampled 4 weeks later. Thirty-nine peaks were detected in the K-31 sample (solid fill); 41 peaks were detected in the K-19 sample. The Dice coefficient of the two samples was 0.975.

FIG. 2

FIG. 2

T-RFs included in the analysis. The sizes of the fragments are in base pairs. Refer to Table 1 for an explanation of the diets (K to G). Solid squares show fragments that were present in samples from the diet groups after 2 weeks of feeding the experimental diets to the animals and before the infection with B. hyodysenteriae. Arrows pointing downward in the “−” column indicate that the fragment disappeared in one or more diet groups after infection. Arrows pointing upward in the “+” column indicate fragments that were first detected in a new diet group after the infection.

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