A simple method for the preparation of large quantities of pure plasmid DNA - PubMed (original) (raw)
A simple method for the preparation of large quantities of pure plasmid DNA
G O Humphreys et al. Biochim Biophys Acta. 1975.
Abstract
Polyethylene glycol quantitatively precipitates plasmid DNA of molecular weight 6-123-10-6, from cleared lysates of plasmid-carrying bacterial strains, After resuspension and density-gradient centrifugation of the precipitated DNA, it is unchanged in length and in transformation efficiency for Escherichia coli K12. Plasmid DNA can be easily prepared in large quantities by including a polyethylene glycol precipitation step in standard plasmid isolation procedures.
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