The Saccharomyces cerevisiae RAD6 group is composed of an error-prone and two error-free postreplication repair pathways - PubMed (original) (raw)

The Saccharomyces cerevisiae RAD6 group is composed of an error-prone and two error-free postreplication repair pathways

W Xiao et al. Genetics. 2000 Aug.

Abstract

The RAD6 postreplication repair and mutagenesis pathway is the only major radiation repair pathway yet to be extensively characterized. It has been previously speculated that the RAD6 pathway consists of two parallel subpathways, one error free and another error prone (mutagenic). Here we show that the RAD6 group genes can be exclusively divided into three rather than two independent subpathways represented by the RAD5, POL30, and REV3 genes; the REV3 pathway is largely mutagenic, whereas the RAD5 and the POL30 pathways are deemed error free. Mutants carrying characteristic mutations in each of the three subpathways are phenotypically indistinguishable from a single mutant such as rad18, which is defective in the entire RAD6 postreplication repair/tolerance pathway. Furthermore, the rad18 mutation is epistatic to all single or combined mutations in any of the above three subpathways. Our data also suggest that MMS2 and UBC13 play a key role in coordinating the response of the error-free subpathways; Mms2 and Ubc13 form a complex required for a novel polyubiquitin chain assembly, which probably serves as a signal transducer to promote both RAD5 and POL30 error-free postreplication repair pathways. The model established by this study will facilitate further research into the molecular mechanisms of postreplication repair and translesion DNA synthesis. In view of the high degree of sequence conservation of the RAD6 pathway genes among all eukaryotes, the model presented in this study may also apply to mammalian cells and predicts links to human diseases.

PubMed Disclaimer

Similar articles

• MMS2, encoding a ubiquitin-conjugating-enzyme-like protein, is a member of the yeast error-free postreplication repair pathway.
  Broomfield S, Chow BL, Xiao W. Broomfield S, et al. Proc Natl Acad Sci U S A. 1998 May 12;95(10):5678-83. doi: 10.1073/pnas.95.10.5678. Proc Natl Acad Sci U S A. 1998. PMID: 9576943 Free PMC article.
• Suppression of genetic defects within the RAD6 pathway by srs2 is specific for error-free post-replication repair but not for damage-induced mutagenesis.
  Broomfield S, Xiao W. Broomfield S, et al. Nucleic Acids Res. 2002 Feb 1;30(3):732-9. doi: 10.1093/nar/30.3.732. Nucleic Acids Res. 2002. PMID: 11809886 Free PMC article.
• Requirement of RAD5 and MMS2 for postreplication repair of UV-damaged DNA in Saccharomyces cerevisiae.
  Torres-Ramos CA, Prakash S, Prakash L. Torres-Ramos CA, et al. Mol Cell Biol. 2002 Apr;22(7):2419-26. doi: 10.1128/MCB.22.7.2419-2426.2002. Mol Cell Biol. 2002. PMID: 11884624 Free PMC article.
• The RAD6 DNA repair pathway in Saccharomyces cerevisiae: what does it do, and how does it do it?
  Lawrence C. Lawrence C. Bioessays. 1994 Apr;16(4):253-8. doi: 10.1002/bies.950160408. Bioessays. 1994. PMID: 8031302 Review.
• The structure and function of RAD6 and RAD18 DNA repair genes of Saccharomyces cerevisiae.
  Prakash L. Prakash L. Genome. 1989;31(2):597-600. doi: 10.1139/g89-111. Genome. 1989. PMID: 2698834 Review.

Cited by

• Mechanisms of PARP1 inhibitor resistance and their implications for cancer treatment.
  Jackson LM, Moldovan GL. Jackson LM, et al. NAR Cancer. 2022 Dec 22;4(4):zcac042. doi: 10.1093/narcan/zcac042. eCollection 2022 Dec. NAR Cancer. 2022. PMID: 36568963 Free PMC article.
• PCNA Ubiquitylation: Instructive or Permissive to DNA Damage Tolerance Pathways?
  Che J, Hong X, Rao H. Che J, et al. Biomolecules. 2021 Oct 19;11(10):1543. doi: 10.3390/biom11101543. Biomolecules. 2021. PMID: 34680175 Free PMC article.
• Temporally distinct post-replicative repair mechanisms fill PRIMPOL-dependent ssDNA gaps in human cells.
  Tirman S, Quinet A, Wood M, Meroni A, Cybulla E, Jackson J, Pegoraro S, Simoneau A, Zou L, Vindigni A. Tirman S, et al. Mol Cell. 2021 Oct 7;81(19):4026-4040.e8. doi: 10.1016/j.molcel.2021.09.013. Mol Cell. 2021. PMID: 34624216 Free PMC article.
• To skip or not to skip: choosing repriming to tolerate DNA damage.
  Quinet A, Tirman S, Cybulla E, Meroni A, Vindigni A. Quinet A, et al. Mol Cell. 2021 Feb 18;81(4):649-658. doi: 10.1016/j.molcel.2021.01.012. Epub 2021 Jan 29. Mol Cell. 2021. PMID: 33515486 Free PMC article. Review.
• The Ubiquitin Conjugating Enzyme: An Important Ubiquitin Transfer Platform in Ubiquitin-Proteasome System.
  Liu W, Tang X, Qi X, Fu X, Ghimire S, Ma R, Li S, Zhang N, Si H. Liu W, et al. Int J Mol Sci. 2020 Apr 21;21(8):2894. doi: 10.3390/ijms21082894. Int J Mol Sci. 2020. PMID: 32326224 Free PMC article. Review.

References

1. 1. Mol Cell Biol. 1997 Aug;17(8):4536-43 - PubMed
2. 1. J Biol Chem. 1997 Sep 12;272(37):23360-5 - PubMed
3. 1. Cell. 1999 Mar 5;96(5):645-53 - PubMed
4. 1. Proc Natl Acad Sci U S A. 1999 Aug 3;96(16):8919-24 - PubMed
5. 1. J Biol Chem. 1997 Sep 26;272(39):24522-9 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • PubMed Central
  • Silverchair Information Systems

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • BioCyc
  • Gene Ontology
  • Saccharomyces Genome Database

• Research Materials

  • NCI CPTC Antibody Characterization Program

• Miscellaneous

  • NCI CPTAC Assay Portal