Involvement of Iba1 in membrane ruffling and phagocytosis of macrophages/microglia - PubMed (original) (raw)
. 2000 Sep:113 ( Pt 17):3073-84.
doi: 10.1242/jcs.113.17.3073.
Affiliations
- PMID: 10934045
- DOI: 10.1242/jcs.113.17.3073
Involvement of Iba1 in membrane ruffling and phagocytosis of macrophages/microglia
K Ohsawa et al. J Cell Sci. 2000 Sep.
Abstract
Ionized calcium binding adaptor molecule 1, Iba1, is an EF hand calcium binding protein whose expression is restricted to macrophages/microglia. In this study, Iba1 was shown to colocalize with F-actin in membrane ruffles induced by macrophage colony-stimulating factor and in phagocytic cups formed during zymosan phagocytosis. Expression of mutant Iba1 carrying either N- or C-terminal deletions or carrying a substitution in the calcium binding domain, suppressed the membrane ruffling and the phagocytosis. These results indicate that Iba1 is a key molecule in membrane ruffling and the phagocytosis of macrophages/microglia. Furthermore, Iba1 colocalized with a small GTPase Rac in the membrane ruffles and the phagocytic cups. The Iba1 mutants also suppressed membrane ruffling induced by dominant active Rac1V12, but do not affect microspikes by Cdc42V12 and stress fibers by RhoAV14. These observations suggest that Iba1 is involved in Rac and calcium signaling pathways.
Similar articles
- Microglia/macrophage-specific protein Iba1 binds to fimbrin and enhances its actin-bundling activity.
Ohsawa K, Imai Y, Sasaki Y, Kohsaka S. Ohsawa K, et al. J Neurochem. 2004 Feb;88(4):844-56. doi: 10.1046/j.1471-4159.2003.02213.x. J Neurochem. 2004. PMID: 14756805 - Macrophage/microglia-specific protein Iba1 enhances membrane ruffling and Rac activation via phospholipase C-gamma -dependent pathway.
Kanazawa H, Ohsawa K, Sasaki Y, Kohsaka S, Imai Y. Kanazawa H, et al. J Biol Chem. 2002 May 31;277(22):20026-32. doi: 10.1074/jbc.M109218200. Epub 2002 Mar 26. J Biol Chem. 2002. PMID: 11916959 - Iba1 is an actin-cross-linking protein in macrophages/microglia.
Sasaki Y, Ohsawa K, Kanazawa H, Kohsaka S, Imai Y. Sasaki Y, et al. Biochem Biophys Res Commun. 2001 Aug 17;286(2):292-7. doi: 10.1006/bbrc.2001.5388. Biochem Biophys Res Commun. 2001. PMID: 11500035 - Intracellular signaling in M-CSF-induced microglia activation: role of Iba1.
Imai Y, Kohsaka S. Imai Y, et al. Glia. 2002 Nov;40(2):164-174. doi: 10.1002/glia.10149. Glia. 2002. PMID: 12379904 Review. - Every day I'm rufflin': Calcium sensing and actin dynamics in the growth factor-independent membrane ruffling of professional phagocytes.
Schlam D, Canton J. Schlam D, et al. Small GTPases. 2017 Apr 3;8(2):65-70. doi: 10.1080/21541248.2016.1197873. Epub 2016 Jun 7. Small GTPases. 2017. PMID: 27267709 Free PMC article. Review.
Cited by
- Immunological Markers for Central Nervous System Glia.
Huang H, He W, Tang T, Qiu M. Huang H, et al. Neurosci Bull. 2023 Mar;39(3):379-392. doi: 10.1007/s12264-022-00938-2. Epub 2022 Aug 26. Neurosci Bull. 2023. PMID: 36028641 Free PMC article. Review. - The Calmodulin-like calcium binding protein EhCaBP3 of Entamoeba histolytica regulates phagocytosis and is involved in actin dynamics.
Aslam S, Bhattacharya S, Bhattacharya A. Aslam S, et al. PLoS Pathog. 2012 Dec;8(12):e1003055. doi: 10.1371/journal.ppat.1003055. Epub 2012 Dec 27. PLoS Pathog. 2012. PMID: 23300437 Free PMC article. - Interferon regulatory factor 8/interferon consensus sequence binding protein is a critical transcription factor for the physiological phenotype of microglia.
Horiuchi M, Wakayama K, Itoh A, Kawai K, Pleasure D, Ozato K, Itoh T. Horiuchi M, et al. J Neuroinflammation. 2012 Sep 28;9:227. doi: 10.1186/1742-2094-9-227. J Neuroinflammation. 2012. PMID: 23020843 Free PMC article. - Targeting Catechol-O-Methyltransferase Induces Mitochondrial Dysfunction and Enhances the Efficacy of Radiotherapy in Glioma.
Jiao M, Pirozzi CJ, Yu C, Bao X, Hu M, Pan D, Littleton S, Reynolds N, Saban DR, Li F, Li CY. Jiao M, et al. Cancer Res. 2024 Nov 4;84(21):3640-3656. doi: 10.1158/0008-5472.CAN-24-0134. Cancer Res. 2024. PMID: 39088832 Free PMC article. - Pten Mutations Alter Brain Growth Trajectory and Allocation of Cell Types through Elevated β-Catenin Signaling.
Chen Y, Huang WC, Séjourné J, Clipperton-Allen AE, Page DT. Chen Y, et al. J Neurosci. 2015 Jul 15;35(28):10252-67. doi: 10.1523/JNEUROSCI.5272-14.2015. J Neurosci. 2015. PMID: 26180201 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous