A novel EH domain protein of Saccharomyces cerevisiae, Ede1p, involved in endocytosis - PubMed (original) (raw)
. 2000 Sep:113 ( Pt 18):3309-19.
doi: 10.1242/jcs.113.18.3309.
Affiliations
- PMID: 10954428
- DOI: 10.1242/jcs.113.18.3309
A novel EH domain protein of Saccharomyces cerevisiae, Ede1p, involved in endocytosis
B Gagny et al. J Cell Sci. 2000 Sep.
Abstract
Sequencing of the entire genome of S. cerevisiae has revealed the existence of five proteins containing EH domains. These are protein-protein interaction modules first described in mammalian Eps15, a protein that is involved in clathrin-dependent endocytosis. Two of the yeast proteins, End3p and Pan1p, are required for the internalization step of endocytosis. We report characterization of the nonessential ORF YBL047c which, like Eps15, encodes a protein with three N-terminal EH domains. Deletion of YBL047c leads to a defective fluid-phase endocytosis and to defective internalization of the pheromone (alpha)-factor and uracil permease. We therefore named YBL047c EDE1, for EH Domains and Endocytosis. Ede1p expressed as a chromosomally encoded fusion to the green fluorescent protein is localized in punctate cortical spots that only partially colocalize with actin patches. This localization is maintained when actin is depolymerized. Deletion of EDE1 impairs the diploid budding pattern, but has only a small impact on actin cytoskeleton organization, in contrast to the effects observed in pan1 cells and many end mutants impaired in proteins colocalizing with cortical actin patches. Genetic interaction was observed between EDE1 and RSP5, which encodes the ubiquitin ligase Rsp5p essential for ubiquitin-dependent endocytosis of many plasma membrane proteins, thus further emphasizing the functional link between Rsp5p and the EH domain proteins. We also observed genetic interaction between EDE1, and END3 or PAN1, suggesting that Ede1p might be part of a yeast EH network implicated in endocytosis.
Similar articles
- Rsp5p, a new link between the actin cytoskeleton and endocytosis in the yeast Saccharomyces cerevisiae.
Kamińska J, Gajewska B, Hopper AK, Zoładek T. Kamińska J, et al. Mol Cell Biol. 2002 Oct;22(20):6946-8. doi: 10.1128/MCB.22.20.6946-6958.2002. Mol Cell Biol. 2002. PMID: 12242276 Free PMC article. - Pan1p, yeast eps15, functions as a multivalent adaptor that coordinates protein-protein interactions essential for endocytosis.
Wendland B, Emr SD. Wendland B, et al. J Cell Biol. 1998 Apr 6;141(1):71-84. doi: 10.1083/jcb.141.1.71. J Cell Biol. 1998. PMID: 9531549 Free PMC article. - Pan1p: an actin director of endocytosis in yeast.
Huang B, Cai M. Huang B, et al. Int J Biochem Cell Biol. 2007;39(10):1760-4. doi: 10.1016/j.biocel.2006.12.001. Epub 2006 Dec 21. Int J Biochem Cell Biol. 2007. PMID: 17303466 Review. - Molecular requirements for the internalisation step of endocytosis: insights from yeast.
Munn AL. Munn AL. Biochim Biophys Acta. 2001 Mar 26;1535(3):236-57. doi: 10.1016/s0925-4439(01)00028-x. Biochim Biophys Acta. 2001. PMID: 11278164 Review.
Cited by
- Roles of CgEde1 and CgMca in Development and Virulence of Colletotrichum gloeosporioides.
Wang D, An B, Luo H, He C, Wang Q. Wang D, et al. Int J Mol Sci. 2024 Mar 3;25(5):2943. doi: 10.3390/ijms25052943. Int J Mol Sci. 2024. PMID: 38474190 Free PMC article. - Wbm0076, a candidate effector protein of the Wolbachia endosymbiont of Brugia malayi, disrupts eukaryotic actin dynamics.
Mills MK, McCabe LG, Rodrigue EM, Lechtreck KF, Starai VJ. Mills MK, et al. PLoS Pathog. 2023 Feb 17;19(2):e1010777. doi: 10.1371/journal.ppat.1010777. eCollection 2023 Feb. PLoS Pathog. 2023. PMID: 36800397 Free PMC article. - Condensation of Ede1 promotes the initiation of endocytosis.
Kozak M, Kaksonen M. Kozak M, et al. Elife. 2022 Apr 12;11:e72865. doi: 10.7554/eLife.72865. Elife. 2022. PMID: 35412456 Free PMC article. - Conserved Ark1-related kinases function in a TORC2 signaling network.
Alcaide-Gavilán M, Lucena R, Banuelos S, Kellogg DR. Alcaide-Gavilán M, et al. Mol Biol Cell. 2020 Aug 15;31(18):2057-2069. doi: 10.1091/mbc.E19-12-0685. Epub 2020 Jul 2. Mol Biol Cell. 2020. PMID: 32614710 Free PMC article. - Identification of Suppressor of Clathrin Deficiency-1 (SCD1) and Its Connection to Clathrin-Mediated Endocytosis in Saccharomyces cerevisiae.
Moorthy BT, Sharma A, Boettner DR, Wilson TE, Lemmon SK. Moorthy BT, et al. G3 (Bethesda). 2019 Mar 7;9(3):867-877. doi: 10.1534/g3.118.200782. G3 (Bethesda). 2019. PMID: 30679249 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Miscellaneous