Coinfection of fibroblasts with Coxiella burnetti and Toxoplasma gondii: to each their own - PubMed (original) (raw)
Coinfection of fibroblasts with Coxiella burnetti and Toxoplasma gondii: to each their own
A P Sinai et al. Microbes Infect. 2000 Jun.
Free article
Abstract
Intracellular pathogens have evolved distinct strategies to subvert host cell defenses. At diametrically opposed ends of the spectrum with regard to the host endosomal/lysosomal defenses are the obligate intracellular protozoan Toxoplasma gondii and the bacterium Coxiella burnetti. While the intracellular replication of T. gondii requires complete avoidance of the host endocytic cascade, C. burnetti actively subverts it. This results in these organisms establishing and growing in very different vacuolar compartments. In this study we examined the potential interaction between these distinct compartments following coinfection of mammalian fibroblasts. When present within the same cell, these organisms exhibit minimal interaction with each other. Colocalization of T. gondii and C. burnetti within the same vacuole occurs at a low frequency in doubly infected cells. In such instances only one of the organisms appears to be replication competent, emphasizing the different requirements for survival and/or intracellular growth. The potential basis for both the lack of interaction between these distinct pathogen-containing compartments, and the mechanisms to address their low frequency of colocalization are discussed in the context of our understanding of the biology of the organisms and membrane traffic in eukaryotic cells.
Similar articles
- Toxoplasma gondii: fusion competence of parasitophorous vacuoles in Fc receptor-transfected fibroblasts.
Joiner KA, Fuhrman SA, Miettinen HM, Kasper LH, Mellman I. Joiner KA, et al. Science. 1990 Aug 10;249(4969):641-6. doi: 10.1126/science.2200126. Science. 1990. PMID: 2200126 - Trypanosoma cruzi cell invasion and traffic: influence of Coxiella burnetii and pH in a comparative study between distinct infective forms.
Fernandes MC, L'Abbate C, Kindro Andreoli W, Mortara RA. Fernandes MC, et al. Microb Pathog. 2007 Jul;43(1):22-36. doi: 10.1016/j.micpath.2007.02.005. Epub 2007 Mar 12. Microb Pathog. 2007. PMID: 17448629 - Determination of stage interconversion in vitro and in vivo by construction of transgenic Toxoplasma gondii that stably express stage-specific fluorescent proteins.
Zhang H, Zhang Y, Cao J, Zhou Y, Wang N, Zhou J. Zhang H, et al. Exp Parasitol. 2013 Jul;134(3):275-80. doi: 10.1016/j.exppara.2013.03.015. Epub 2013 Mar 29. Exp Parasitol. 2013. PMID: 23545429 - [Formation mechanism and the function of parasitophorous vacuole of Toxoplasma gondii].
Peng HJ. Peng HJ. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2010 Oct 30;28(5):382-6. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2010. PMID: 21351554 Review. Chinese. - Interactions between Toxoplasma gondii and its mammalian host cells.
Sibley LD. Sibley LD. Semin Cell Biol. 1993 Oct;4(5):335-44. doi: 10.1006/scel.1993.1040. Semin Cell Biol. 1993. PMID: 8257785 Review.
Cited by
- The opposing effect of acute and chronic Toxoplasma gondii infection on tumor development.
Song Y, Yuan H, Yang X, Yang Z, Ren Z, Qi S, He H, Zhang XX, Jiang T, Yuan ZG. Song Y, et al. Parasit Vectors. 2024 Jun 4;17(1):247. doi: 10.1186/s13071-024-06240-6. Parasit Vectors. 2024. PMID: 38835064 Free PMC article. - Trypanosoma cruzi Differentiates and Multiplies within Chimeric Parasitophorous Vacuoles in Macrophages Coinfected with Leishmania amazonensis.
Pessoa CC, Ferreira ÉR, Bayer-Santos E, Rabinovitch M, Mortara RA, Real F. Pessoa CC, et al. Infect Immun. 2016 Apr 22;84(5):1603-1614. doi: 10.1128/IAI.01470-15. Print 2016 May. Infect Immun. 2016. PMID: 26975994 Free PMC article. - Novel Approaches Reveal that Toxoplasma gondii Bradyzoites within Tissue Cysts Are Dynamic and Replicating Entities In Vivo.
Watts E, Zhao Y, Dhara A, Eller B, Patwardhan A, Sinai AP. Watts E, et al. mBio. 2015 Sep 8;6(5):e01155-15. doi: 10.1128/mBio.01155-15. mBio. 2015. PMID: 26350965 Free PMC article. - Host Organelle Hijackers: a similar modus operandi for Toxoplasma gondii and Chlamydia trachomatis: co-infection model as a tool to investigate pathogenesis.
Romano JD, Coppens I. Romano JD, et al. Pathog Dis. 2013 Nov;69(2):72-86. doi: 10.1111/2049-632X.12057. Epub 2013 Jul 22. Pathog Dis. 2013. PMID: 23821471 Free PMC article. Review. - Fierce competition between Toxoplasma and Chlamydia for host cell structures in dually infected cells.
Romano JD, de Beaumont C, Carrasco JA, Ehrenman K, Bavoil PM, Coppens I. Romano JD, et al. Eukaryot Cell. 2013 Feb;12(2):265-77. doi: 10.1128/EC.00313-12. Epub 2012 Dec 14. Eukaryot Cell. 2013. PMID: 23243063 Free PMC article.