Intracellular accumulation of polyphosphate by the yeast Candida humicola G-1 in response to acid pH - PubMed (original) (raw)

Intracellular accumulation of polyphosphate by the yeast Candida humicola G-1 in response to acid pH

J W McGrath et al. Appl Environ Microbiol. 2000 Sep.

Abstract

Cells of a newly isolated environmental strain of Candida humicola accumulated 10-fold more polyphosphate (polyP), during active growth, when grown in complete glucose-mineral salts medium at pH 5.5 than when grown at pH 7.5. Neither phosphate starvation, nutrient limitation, nor anaerobiosis was required to induce polyP formation. An increase in intracellular polyP was accompanied by a 4.5-fold increase in phosphate uptake from the medium and sixfold-higher levels of cellular polyphosphate kinase activity. This novel accumulation of polyP by C. humicola G-1 in response to acid pH provides further evidence as to the importance of polyP in the physiological adaptation of microbial cells during growth and development and in their response to environmental stresses.

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Figures

FIG. 1

FIG. 1

(A) Growth and intracellular polyP accumulation by C. humicola G-1 at pH 5.5. (B) Stained cells of C. humicola G-1 grown at pH 5.5, showing polyP granules stained blue-black with Neisser stain. Magnification, ×1,000.

FIG. 1

FIG. 1

(A) Growth and intracellular polyP accumulation by C. humicola G-1 at pH 5.5. (B) Stained cells of C. humicola G-1 grown at pH 5.5, showing polyP granules stained blue-black with Neisser stain. Magnification, ×1,000.

FIG. 2

FIG. 2

(A) Growth and intracellular polyP accumulation by C. humicola G-1 at pH 7.5. (B) Stained cells of C. humicola G-1 grown at pH 7.5, showing the absence of polyP granules. Magnification, ×1,000.

FIG. 2

FIG. 2

(A) Growth and intracellular polyP accumulation by C. humicola G-1 at pH 7.5. (B) Stained cells of C. humicola G-1 grown at pH 7.5, showing the absence of polyP granules. Magnification, ×1,000.

FIG. 3

FIG. 3

(A) Growth, glucose mineralization, and phosphate removal by C. humicola G-1 at pH 5.5. (B) Growth, glucose mineralization, and phosphate removal by C. humicola G-1 at pH 7.5.

FIG. 4

FIG. 4

Phosphate uptake by C. humicola G-1 at pH 4.5 to 7.5.

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