An analytical system for rapid separation of tissue nucleotides at low pressures on conventional anion exchangers - PubMed (original) (raw)

. 1975 Aug;21(9):1245-52.

• PMID: 1097132

An analytical system for rapid separation of tissue nucleotides at low pressures on conventional anion exchangers

J X Khym. Clin Chem. 1975 Aug.

Abstract

An analytical anion-exchange procedure has been developed for the rapid separation of acid-soluble nucleotides (the so-called "free" or tissue nucleotides). It permits assay at low pressures (275-415 kPa; 40-60 psi) in less than 1 h on 10-cm columns of Aminex resins (conventional styrene-type anion exchangers) with alkaline citrate solutions as the eluent. Separation variables have been investigated, to determine optimum conditions for the routine analysis of samples containing tissue nucleotides. Also described here is a simple solvent-extraction procedure for removing HCIO4 or CCI3CO2H quantitatively from cell extracts that contain acid-soluble nucleotides: they are removed from aqueous acid solutions with a water-insoluble amine dissolved in a water-immiscible solvent.

PubMed Disclaimer

Similar articles

• Separation of oligonucleotides, nucleotides, and nucleosides on columns of polystyrene anion-exchangers with solvent systems containing ethanol.
  Asteriadis GT, Armbruster MA, Gilham PT. Asteriadis GT, et al. Anal Biochem. 1976 Jan;70(1):64-74. doi: 10.1016/s0003-2697(76)80048-6. Anal Biochem. 1976. PMID: 1259157 No abstract available.
• Rapid separation of tissue nucleotides on conventional anion-exchange resins at low pressures.
  Khym JX. Khym JX. J Chromatogr. 1974 Oct 23;97(2):277-9. doi: 10.1016/s0021-9673(00)95607-3. J Chromatogr. 1974. PMID: 4426960 No abstract available.
• Anion-exchange chromatography on reversed-phase columns: isolation and assay of nucleosides, nucleotides, and oligonucleotides from nucleic acids and from cytoplasm.
  Singhal RP. Singhal RP. Biochim Biophys Acta. 1973 Aug 10;319(1):11-24. doi: 10.1016/0005-2787(73)90036-1. Biochim Biophys Acta. 1973. PMID: 4354797 No abstract available.
• High-performance ion-exchange chromatography with narrow-bore columns: rapid analysis of nucleic acid constituents at the subnanomole level.
  Horvath C. Horvath C. Methods Biochem Anal. 1973;21:79-154. doi: 10.1002/9780470110416.ch3. Methods Biochem Anal. 1973. PMID: 4594522 Review. No abstract available.

Cited by

• Osmoregulatory changes in myo-inositol transport by renal cells.
  Nakanishi T, Turner RJ, Burg MB. Nakanishi T, et al. Proc Natl Acad Sci U S A. 1989 Aug;86(15):6002-6. doi: 10.1073/pnas.86.15.6002. Proc Natl Acad Sci U S A. 1989. PMID: 2762310 Free PMC article.
• Antiviral activities of guanosine analogs in guinea pig embryonic fibroblasts.
  Harmenberg J, Stenberg K. Harmenberg J, et al. Antimicrob Agents Chemother. 1988 Oct;32(10):1533-6. doi: 10.1128/AAC.32.10.1533. Antimicrob Agents Chemother. 1988. PMID: 2847633 Free PMC article.
• Factors that influence the therapeutic activity of 5-fluorouracil [6RS]leucovorin combinations in colon adenocarcinoma xenografts.
  Houghton JA, Williams LG, Loftin SK, Cheshire PJ, Morton CL, Houghton PJ, Dayan A, Jolivet J. Houghton JA, et al. Cancer Chemother Pharmacol. 1992;30(6):423-32. doi: 10.1007/BF00685592. Cancer Chemother Pharmacol. 1992. PMID: 1394798
• A novel cycling assay for cellular cADP-ribose with nanomolar sensitivity.
  Graeff R, Lee HC. Graeff R, et al. Biochem J. 2002 Jan 15;361(Pt 2):379-84. doi: 10.1042/bj3610379. Biochem J. 2002. PMID: 11772410 Free PMC article.
• Energy status and functioning of phosphorus-deficient soybean nodules.
  Sa TM, Israel DW. Sa TM, et al. Plant Physiol. 1991 Nov;97(3):928-35. doi: 10.1104/pp.97.3.928. Plant Physiol. 1991. PMID: 16668533 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Other Literature Sources

  • The Lens - Patent Citations Database