Episomal vectors for gene expression in mammalian cells - PubMed (original) (raw)
Review
Episomal vectors for gene expression in mammalian cells
K Van Craenenbroeck et al. Eur J Biochem. 2000 Sep.
Free article
Abstract
An important reason for preferring mammalian cells for heterologous gene expression is their ability to make authentic proteins containing post-translational modifications similar to those of the native protein. The development of expression systems for mammalian cells has been ongoing for several years, resulting in a wide variety of effective expression vectors. The aim of this review is to highlight episomal expression vectors. Such episomal plasmids are usually based on sequences from DNA viruses, such as BK virus, bovine papilloma virus 1 and Epstein-Barr virus. In this review we will mainly focus on the improvements made towards the usefulness of these systems for gene expression studies and gene therapy.
Similar articles
- Molecular integrity and usefulness of episomal expression vectors derived from BK and Epstein-Barr virus.
Van Craenenbroeck K, Vanhoenacker P, Duchau H, Haegeman G. Van Craenenbroeck K, et al. Gene. 2000 Aug 8;253(2):293-301. doi: 10.1016/s0378-1119(00)00242-0. Gene. 2000. PMID: 10940567 - Infectious Epstein-Barr virus vectors for episomal gene therapy.
Wang J, Vos JM. Wang J, et al. Methods Enzymol. 2002;346:649-60. doi: 10.1016/s0076-6879(02)46083-1. Methods Enzymol. 2002. PMID: 11883097 No abstract available. - Development of a novel helper-dependent adenovirus-Epstein-Barr virus hybrid system for the stable transformation of mammalian cells.
Dorigo O, Gil JS, Gallaher SD, Tan BT, Castro MG, Lowenstein PR, Calos MP, Berk AJ. Dorigo O, et al. J Virol. 2004 Jun;78(12):6556-66. doi: 10.1128/JVI.78.12.6556-6566.2004. J Virol. 2004. PMID: 15163748 Free PMC article. - Epstein-Barr virus vectors for gene therapy.
Komaki S, Vos JM. Komaki S, et al. Adv Virus Res. 2000;55:453-62. doi: 10.1016/s0065-3527(00)55012-x. Adv Virus Res. 2000. PMID: 11050951 Review. No abstract available. - Molecular genetic analysis of herpesviruses and their potential use as vectors for gene therapy applications.
Cotter MA, Robertson ES. Cotter MA, et al. Curr Opin Mol Ther. 1999 Oct;1(5):633-44. Curr Opin Mol Ther. 1999. PMID: 11249670 Review.
Cited by
- Exploring the potential of extrachromosomal DNA as a novel oncogenic driver.
Zhu H, Huangfu L, Chen J, Ji J, Xing X. Zhu H, et al. Sci China Life Sci. 2025 Jan;68(1):144-157. doi: 10.1007/s11427-024-2710-3. Epub 2024 Sep 27. Sci China Life Sci. 2025. PMID: 39349791 Review. - Plasmid-Based Generation of Induced Neural Stem Cells from Adult Human Fibroblasts.
Capetian P, Azmitia L, Pauly MG, Krajka V, Stengel F, Bernhardi EM, Klett M, Meier B, Seibler P, Stanslowsky N, Moser A, Knopp A, Gillessen-Kaesbach G, Nikkhah G, Wegner F, Döbrössy M, Klein C. Capetian P, et al. Front Cell Neurosci. 2016 Oct 24;10:245. doi: 10.3389/fncel.2016.00245. eCollection 2016. Front Cell Neurosci. 2016. PMID: 27822179 Free PMC article. - Functional complementation of a genetic deficiency with human artificial chromosomes.
Mejía JE, Willmott A, Levy E, Earnshaw WC, Larin Z. Mejía JE, et al. Am J Hum Genet. 2001 Aug;69(2):315-26. doi: 10.1086/321977. Epub 2001 Jul 10. Am J Hum Genet. 2001. PMID: 11452360 Free PMC article. - Large-scale transient transfection of mammalian cells: a newly emerging attractive option for recombinant protein production.
Geisse S, Henke M. Geisse S, et al. J Struct Funct Genomics. 2005;6(2-3):165-70. doi: 10.1007/s10969-005-2826-4. J Struct Funct Genomics. 2005. PMID: 16211514 - Characterizing Endogenous Protein Complexes with Biological Mass Spectrometry.
Rogawski R, Sharon M. Rogawski R, et al. Chem Rev. 2022 Apr 27;122(8):7386-7414. doi: 10.1021/acs.chemrev.1c00217. Epub 2021 Aug 18. Chem Rev. 2022. PMID: 34406752 Free PMC article. Review.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical