Electron microscopic examination of subcellular fractions. I. The preparation of representative samples from suspensions of particles - PubMed (original) (raw)
Electron microscopic examination of subcellular fractions. I. The preparation of representative samples from suspensions of particles
P Baudhuin et al. J Cell Biol. 1967 Jan.
Abstract
A method is described for preparing, by filtration on Millipore filters, very thin (about 10 micro) pellicles of packed particles. These pellicles can be embedded in Epon for electron microscopic examination. They are also suitable for cytochemical assays. The method was used with various particulate fractions from rat liver. Its main advantages over the usual centrifugal packing techniques are that it produces heterogeneity solely in the direction perpendicular to the surface of the pellicle and that sections covering the whole depth of the pellicle can be photographed in a single field. It thus answers the essential criterion of random sampling and can be used for accurate quantitative evaluations.
Similar articles
- Preparation of representative samples of subcellular fractions for electron microscopy by filtration with dextran.
Palade P, Saito A, Mitchell RD, Fleischer S. Palade P, et al. J Histochem Cytochem. 1983 Jul;31(7):971-4. doi: 10.1177/31.7.6189886. J Histochem Cytochem. 1983. PMID: 6189886 - Rapid three-dimensional reconstruction at the light microscopic level and a technique for re-embedding the same semithin sections for electron microscopic examination.
Pignot-Paintrand I, Bressac C. Pignot-Paintrand I, et al. Biotech Histochem. 1992 Jan;67(1):55-7. doi: 10.3109/10520299209110007. Biotech Histochem. 1992. PMID: 1617002 - A rapid method for embedding fractionated samples for electron microscopy.
Takagi I, Sato T, Yamada K. Takagi I, et al. J Electron Microsc (Tokyo). 1979;28(4):316-9. J Electron Microsc (Tokyo). 1979. PMID: 536664 No abstract available. - Artifacts caused by dehydration and epoxy embedding in transmission electron microscopy.
Mollenhauer HH. Mollenhauer HH. Microsc Res Tech. 1993 Dec 15;26(6):496-512. doi: 10.1002/jemt.1070260604. Microsc Res Tech. 1993. PMID: 8305727 Review. - [Extramitochondrial oxidative systems].
Rubin BA, Loginova LN. Rubin BA, et al. Usp Sovrem Biol. 1971 Sep-Oct;72(2):253-72. Usp Sovrem Biol. 1971. PMID: 4336477 Review. Russian. No abstract available.
Cited by
- Expression, sorting, and segregation of Golgi proteins during germ cell differentiation in the testis.
Au CE, Hermo L, Byrne E, Smirle J, Fazel A, Simon PH, Kearney RE, Cameron PH, Smith CE, Vali H, Fernandez-Rodriguez J, Ma K, Nilsson T, Bergeron JJ. Au CE, et al. Mol Biol Cell. 2015 Nov 5;26(22):4015-32. doi: 10.1091/mbc.E14-12-1632. Epub 2015 Mar 25. Mol Biol Cell. 2015. PMID: 25808494 Free PMC article. - Detection of GTP-binding proteins in purified derivatives of rough endoplasmic reticulum.
Lanoix J, Roy L, Paiement J. Lanoix J, et al. Biochem J. 1989 Sep 1;262(2):497-503. doi: 10.1042/bj2620497. Biochem J. 1989. PMID: 2508629 Free PMC article. - Synthesis of rat liver microsomal cytochrome b5 by free ribosomes.
Rachubinski RA, Verma DP, Bergeron JJ. Rachubinski RA, et al. J Cell Biol. 1980 Mar;84(3):705-16. doi: 10.1083/jcb.84.3.705. J Cell Biol. 1980. PMID: 7358795 Free PMC article. - Isolation and characterization of granules of the toad bladder.
Masur SK, Cooper S, Massardo S, Gronowicz G, Rubin MS. Masur SK, et al. J Membr Biol. 1986;89(1):39-51. doi: 10.1007/BF01870894. J Membr Biol. 1986. PMID: 2937922 - Tissue fraction-past and present.
de Duve C. de Duve C. J Cell Biol. 1971 Jul 1;50(1):20. J Cell Biol. 1971. PMID: 19866786 Free PMC article. No abstract available.
References
- Arch Int Physiol Biochim. 1964 Sep;72(4):698-700 - PubMed
- J Theor Biol. 1964 Jan;6(1):33-59 - PubMed
- J Biophys Biochem Cytol. 1961 Feb;9:409-14 - PubMed
- J Cell Biol. 1963 Apr;17:208-12 - PubMed
- J Cell Biol. 1964 Dec;23:519-52 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources