Reconstitution of apo-superoxide dismutase by nitric oxide-induced copper transfer from metallothioneins - PubMed (original) (raw)
Reconstitution of apo-superoxide dismutase by nitric oxide-induced copper transfer from metallothioneins
S X Liu et al. Chem Res Toxicol. 2000 Sep.
Abstract
Little is known about copper transfer from Cu-metallothionein (Cu-MT) to various target proteins, such as apo-SOD, and the potential role of redox mechanisms in this transfer. We studied Cu transfer from Cu-MT to apo/Zn-SOD in a cell-free model system and found that Cu(5)-MT and Cu(10)-MT were able to reconstitute SOD activity only in the presence of a nitric oxide donor, (Z)-[N-(3-ammoniopropyl)-N-(n-propyl)amino]diazen-1-ium++ +-1,2-diolate (NOC-15). The percentage of reconstitution by Cu(5)-MT and Cu(10)-MT was 34 and 83%, respectively, compared with that reconstituted by free Cu alone. A Cu chelation assay using bathocuproine disulfonate (BCS) showed that NOC-15 induced release of free Cu from Cu(10)-MT but not from Cu(5)-MT. The transfer of Cu from Cu-MT to apo/Zn-SOD was not accompanied by enhanced Cu-dependent generation of ascorbate radicals or hydroxyl radicals as measured by EPR spectroscopy. We found a 70% decrease in the number of 2,2'-dithiodipyridine titratable SH groups on MT after incubation with NOC-15. Overall, our results suggest that Cu-MT could potentially function in a nitric oxide-dependent pathway for the delivery of Cu to apo-SOD in copper-challenged cells.
Similar articles
- Bifunctional anti/prooxidant potential of metallothionenin: redox signaling of copper binding and release.
Fabisiak JP, Pearce LL, Borisenko GG, Tyhurina YY, Tyurin VA, Razzack J, Lazo JS, Pitt BR, Kagan VE. Fabisiak JP, et al. Antioxid Redox Signal. 1999 Fall;1(3):349-64. doi: 10.1089/ars.1999.1.3-349. Antioxid Redox Signal. 1999. PMID: 11229446 - Redox regulation of copper-metallothionein.
Fabisiak JP, Tyurin VA, Tyurina YY, Borisenko GG, Korotaeva A, Pitt BR, Lazo JS, Kagan VE. Fabisiak JP, et al. Arch Biochem Biophys. 1999 Mar 1;363(1):171-81. doi: 10.1006/abbi.1998.1077. Arch Biochem Biophys. 1999. PMID: 10049512 - Metallothionein in human disease.
Simpkins CO. Simpkins CO. Cell Mol Biol (Noisy-le-grand). 2000 Mar;46(2):465-88. Cell Mol Biol (Noisy-le-grand). 2000. PMID: 10774934 Review. - Metallothionein and apoptosis in the toxic milk mutant mouse.
Deng DX, Ono S, Koropatnick J, Cherian MG. Deng DX, et al. Lab Invest. 1998 Feb;78(2):175-83. Lab Invest. 1998. PMID: 9484715 Review.
Cited by
- Trichoderma viride Colonizes the Roots of Brassica napus L., Alters the Expression of Stress-Responsive Genes, and Increases the Yield of Canola under Field Conditions during Drought.
Garstecka Z, Antoszewski M, Mierek-Adamska A, Krauklis D, Niedojadło K, Kaliska B, Hrynkiewicz K, Dąbrowska GB. Garstecka Z, et al. Int J Mol Sci. 2023 Oct 19;24(20):15349. doi: 10.3390/ijms242015349. Int J Mol Sci. 2023. PMID: 37895028 Free PMC article. - Redox metals homeostasis in multiple sclerosis and amyotrophic lateral sclerosis: a review.
Sheykhansari S, Kozielski K, Bill J, Sitti M, Gemmati D, Zamboni P, Singh AV. Sheykhansari S, et al. Cell Death Dis. 2018 Mar 1;9(3):348. doi: 10.1038/s41419-018-0379-2. Cell Death Dis. 2018. PMID: 29497049 Free PMC article. Review. - NO-inducible nitrosothionein mediates NO removal in tandem with thioredoxin.
Zhou S, Narukami T, Masuo S, Shimizu M, Fujita T, Doi Y, Kamimura Y, Takaya N. Zhou S, et al. Nat Chem Biol. 2013 Oct;9(10):657-63. doi: 10.1038/nchembio.1316. Epub 2013 Aug 18. Nat Chem Biol. 2013. PMID: 23955366 - Functional role of intracellular labile zinc in pulmonary endothelium.
Thambiayya K, Kaynar AM, St Croix CM, Pitt BR. Thambiayya K, et al. Pulm Circ. 2012 Oct;2(4):443-51. doi: 10.4103/2045-8932.105032. Pulm Circ. 2012. PMID: 23372928 Free PMC article. - Metallothionein-induced zinc partitioning exacerbates hyperoxic acute lung injury.
Lee SM, McLaughlin JN, Frederick DR, Zhu L, Thambiayya K, Wasserloos KJ, Kaminski I, Pearce LL, Peterson J, Li J, Latoche JD, Peck Palmer OM, Stolz DB, Fattman CL, Alcorn JF, Oury TD, Angus DC, Pitt BR, Kaynar AM. Lee SM, et al. Am J Physiol Lung Cell Mol Physiol. 2013 Mar 1;304(5):L350-60. doi: 10.1152/ajplung.00243.2012. Epub 2012 Dec 28. Am J Physiol Lung Cell Mol Physiol. 2013. PMID: 23275622 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources