LEAP-1, a novel highly disulfide-bonded human peptide, exhibits antimicrobial activity - PubMed (original) (raw)
LEAP-1, a novel highly disulfide-bonded human peptide, exhibits antimicrobial activity
A Krause et al. FEBS Lett. 2000.
Free article
Abstract
We report the isolation and characterization of a novel human peptide with antimicrobial activity, termed LEAP-1 (liver-expressed antimicrobial peptide). Using a mass spectrometric assay detecting cysteine-rich peptides, a 25-residue peptide containing four disulfide bonds was identified in human blood ultrafiltrate. LEAP-1 expression was predominantly detected in the liver, and, to a much lower extent, in the heart. In radial diffusion assays, Gram-positive Bacillus megaterium, Bacillus subtilis, Micrococcus luteus, Staphylococcus carnosus, and Gram-negative Neisseria cinerea as well as the yeast Saccharomyces cerevisiae dose-dependently exhibited sensitivity upon treatment with synthetic LEAP-1. The discovery of LEAP-1 extends the known families of mammalian peptides with antimicrobial activity by its novel disulfide motif and distinct expression pattern.
Similar articles
- Isolation and biochemical characterization of LEAP-2, a novel blood peptide expressed in the liver.
Krause A, Sillard R, Kleemeier B, Klüver E, Maronde E, Conejo-García JR, Forssmann WG, Schulz-Knappe P, Nehls MC, Wattler F, Wattler S, Adermann K. Krause A, et al. Protein Sci. 2003 Jan;12(1):143-52. doi: 10.1110/ps.0213603. Protein Sci. 2003. PMID: 12493837 Free PMC article. - Structure-activity relationship of human liver-expressed antimicrobial peptide 2.
Hocquellet A, Odaert B, Cabanne C, Noubhani A, Dieryck W, Joucla G, Le Senechal C, Milenkov M, Chaignepain S, Schmitter JM, Claverol S, Santarelli X, Dufourc EJ, Bonneu M, Garbay B, Costaglioli P. Hocquellet A, et al. Peptides. 2010 Jan;31(1):58-66. doi: 10.1016/j.peptides.2009.10.006. Epub 2009 Oct 21. Peptides. 2010. PMID: 19852990 - Human natriuretic peptides exhibit antimicrobial activity.
Krause A, Liepke C, Meyer M, Adermann K, Forssmann WG, Maronde E. Krause A, et al. Eur J Med Res. 2001 May 29;6(5):215-8. Eur J Med Res. 2001. PMID: 11410403 - Penaeidins, a family of antimicrobial peptides from penaeid shrimp (Crustacea, Decapoda).
Destoumieux D, Munoz M, Bulet P, Bachère E. Destoumieux D, et al. Cell Mol Life Sci. 2000 Aug;57(8-9):1260-71. doi: 10.1007/pl00000764. Cell Mol Life Sci. 2000. PMID: 11028917 Free PMC article. Review. - The role of cationic antimicrobial peptides in innate host defences.
Hancock RE, Diamond G. Hancock RE, et al. Trends Microbiol. 2000 Sep;8(9):402-10. doi: 10.1016/s0966-842x(00)01823-0. Trends Microbiol. 2000. PMID: 10989307 Review.
Cited by
- Liver-expressed antimicrobial peptide 2 is a hepatokine regulated by ghrelin, nutrients, and body weight.
Islam MN, Nabekura H, Ueno H, Nishida T, Nanashima A, Sakoda H, Zhang W, Nakazato M. Islam MN, et al. Sci Rep. 2024 Oct 21;14(1):24782. doi: 10.1038/s41598-024-74048-6. Sci Rep. 2024. PMID: 39433849 Free PMC article. - Exploring the neuroprotective potential of KC14 peptide from Cyprinus carpio against oxidative stress-induced neurodegeneration by regulating antioxidant mechanism.
Vijayanand M, Issac PK, Velayutham M, Shaik MR, Hussain SA, Guru A. Vijayanand M, et al. Mol Biol Rep. 2024 Sep 17;51(1):990. doi: 10.1007/s11033-024-09905-8. Mol Biol Rep. 2024. PMID: 39287730 - Insights into Iron Metabolism Parameters in Ischemic Stroke: A Single-Center Prospective Cohort Study.
Boinska J, Słomka A, Sury M, Wiszniewska M, Pisarek E, Żekanowska E. Boinska J, et al. Int J Mol Sci. 2024 Aug 29;25(17):9352. doi: 10.3390/ijms25179352. Int J Mol Sci. 2024. PMID: 39273300 Free PMC article. - Functional iron deficiency anemia in patients with cancer.
Koh JS, Song IC. Koh JS, et al. Blood Res. 2024 Aug 7;59(1):26. doi: 10.1007/s44313-024-00030-w. Blood Res. 2024. PMID: 39110268 Free PMC article. Review. - Hepcidin, in contrast to heparin binding protein, does not portend acute kidney injury in patients with community acquired septic shock.
Olinder J, Stjernqvist MJ, Lindén A, Salomonsson ET, Annborn M, Herwald H, Rydén C. Olinder J, et al. PLoS One. 2024 May 2;19(5):e0299257. doi: 10.1371/journal.pone.0299257. eCollection 2024. PLoS One. 2024. PMID: 38696394 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases