Anti-atherogenic effect of coenzyme Q10 in apolipoprotein E gene knockout mice - PubMed (original) (raw)

Anti-atherogenic effect of coenzyme Q10 in apolipoprotein E gene knockout mice

P K Witting et al. Free Radic Biol Med. 2000 Aug.

Abstract

Oxidation of low-density lipoprotein (LDL) lipid is implicated in atherogenesis and certain antioxidants inhibit atherosclerosis. Ubiquinol-10 (CoQ10H2) inhibits LDL lipid peroxidation in vitro although it is not known whether such activity occurs in vivo, and, if so, whether this is anti-atherogenic. We therefore tested the effect of ubiquinone-10 (CoQ10) supplemented at 1% (w/w) on aortic lipoprotein lipid peroxidation and atherosclerosis in apolipoprotein E-deficient (apoE-/-) mice fed a high-fat diet. Hydroperoxides of cholesteryl esters and triacylglycerols (together referred to as LOOH) and their corresponding alcohols were used as the marker for lipoprotein lipid oxidation. Atherosclerosis was assessed by morphometry at the aortic root, proximal and distal arch, and the descending thoracic and abdominal aorta. Compared to controls, CoQ10-treatment increased plasma coenzyme Q, ascorbate, and the CoQ10H2:CoQ10 + CoQ10H2 ratio, decreased plasma alpha-tocopherol (alpha-TOH), and had no effect on cholesterol and cholesterylester alcohols (CE-OH). Plasma from CoQ10-supplemented mice was more resistant to ex vivo lipid peroxidation. CoQ10 treatment increased aortic coenzyme Q and alpha-TOH and decreased the absolute concentration of LOOH, whereas tissue cholesterol, cholesteryl esters, CE-OH, and LOOH expressed per bisallylic hydrogen-containing lipids were not significantly different. CoQ10-treatment significantly decreased lesion size in the aortic root and the ascending and the descending aorta. Together these data show that CoQ10 decreases the absolute concentration of aortic LOOH and atherosclerosis in apoE-/- mice.

PubMed Disclaimer

Cited by

Antioxidants and Atherosclerosis: Mechanistic Aspects.
Malekmohammad K, Sewell RDE, Rafieian-Kopaei M. Malekmohammad K, et al. Biomolecules. 2019 Jul 25;9(8):301. doi: 10.3390/biom9080301. Biomolecules. 2019. PMID: 31349600 Free PMC article. Review.
Formation of Food Grade Microemulsion with Rice Glycosphingolipids to Enhance the Oral Absorption of Coenzyme Q10.
Uchiyama H, Chae J, Kadota K, Tozuka Y. Uchiyama H, et al. Foods. 2019 Oct 15;8(10):502. doi: 10.3390/foods8100502. Foods. 2019. PMID: 31618946 Free PMC article.
Ferroptosis Signaling and Regulators in Atherosclerosis.
Wang Y, Zhao Y, Ye T, Yang L, Shen Y, Li H. Wang Y, et al. Front Cell Dev Biol. 2021 Dec 16;9:809457. doi: 10.3389/fcell.2021.809457. eCollection 2021. Front Cell Dev Biol. 2021. PMID: 34977044 Free PMC article. Review.
Bioenergetic and antioxidant properties of coenzyme Q10: recent developments.
Littarru GP, Tiano L. Littarru GP, et al. Mol Biotechnol. 2007 Sep;37(1):31-7. doi: 10.1007/s12033-007-0052-y. Mol Biotechnol. 2007. PMID: 17914161 Review.
Coenzyme Q10 increases cholesterol efflux and inhibits atherosclerosis through microRNAs.
Allen RM, Vickers KC. Allen RM, et al. Arterioscler Thromb Vasc Biol. 2014 Sep;34(9):1795-7. doi: 10.1161/ATVBAHA.114.303741. Arterioscler Thromb Vasc Biol. 2014. PMID: 25142877 Free PMC article.

Anti-atherogenic effect of coenzyme Q10 in apolipoprotein E gene knockout mice - PubMed (original) (raw)