A viral member of the ERV1/ALR protein family participates in a cytoplasmic pathway of disulfide bond formation - PubMed (original) (raw)

Multiple alignment of the ERV1/ALR protein family. The protein designations consist of the Gene Identification (GI) numbers, gene names, and abbreviated species names. The positions of the aligned amino acid residues in each sequence are indicated in front of the sequences. The lengths of the poorly conserved spacers between the aligned regions are indicated. The lymphocystis disease virus (LDV) homolog of E10R and Plasmodium falciparum homolog of ERV1 are not annotated as proteins in the GenBank database and were identified by searching the corresponding genome sequences (L63545 and AL031745) using the

tblastn

program. The consensus derived by using the 90% conservation is shown underneath the alignment; b indicates “big” residues (E, K, R, I, L, M, F, Y, and W), h indicates hydrophobic residues (A, C, F, I, L, M, V, W, and Y), s indicates small residues (A, C, S, T, D, N, V, G, and P), and p indicates polar residues (D, E, H, K, N, Q, R, S, and T). The conserved cysteines predicted to form a redox-active center are highlighted by reverse shading. The multiple-alignment-based secondary structure prediction is shown on top of the alignment; H (h) indicates α-helix (uppercase indicates the most confident prediction). E10Rh and ERV1h refer to homologs of E10R and ERV1. Species abbreviations: VAC, vaccinia virus; RFV, rabbit fibroma virus; MYX, myxoma virus; MCV, molluscum contagiosum virus; FPV, fowlpox virus; MSV, Melanoplus sanguinipes entomopoxvirus; AFSV, African swine fever virus; LDV1, lymphocystis disease virus 1 (an iridovirus); PBCV1,Paramecium bursaria Chlorella virus 1; Sce,Saccharomyces cerevisiae, Spo, Schizosaccharomyces pombe; Hsa, Homo sapiens; Cel,Caenorhabditis elegans; Dme,Drosophila melanogaster; Lma, Leishmania major; Pfa, Plasmodium falciparum; Ath, Arabidopsis thaliana.