Quantitative gene expression analysis in microdissected archival tissue by real-time RT-PCR - PubMed (original) (raw)
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- PMID: 11043388
Quantitative gene expression analysis in microdissected archival tissue by real-time RT-PCR
K Specht et al. J Mol Med (Berl). 2000.
Abstract
Laser-assisted microdissection is a powerful tool for the analysis of morphologically defined cell populations. We report here that the combination of microdissection and real-time RT-PCR technologies together with an optimized RNA microscale extraction procedure allows the reproducible determination of gene expression levels in archival formalin-fixed, paraffin-embedded tissue samples.
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