Quantitation of Pseudomonas aeruginosa in wound biopsy samples: from bacterial culture to rapid 'real-time' polymerase chain reaction - PubMed (original) (raw)

Comparative Study

doi: 10.1186/cc702. Epub 2000 Jul 7.

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Comparative Study

Quantitation of Pseudomonas aeruginosa in wound biopsy samples: from bacterial culture to rapid 'real-time' polymerase chain reaction

J P Pirnay et al. Crit Care. 2000.

Abstract

STATEMENT OF FINDINGS: We developed a real-time detection (RTD) polymerase chain reaction (PCR) with rapid thermal cycling to detect and quantify Pseudomonas aeruginosa in wound biopsy samples. This method produced a linear quantitative detection range of 7 logs, with a lower detection limit of 103 colony-forming units (CFU)/g tissue or a few copies per reaction. The time from sample collection to result was less than 1h. RTD-PCR has potential for rapid quantitative detection of pathogens in critical care patients, enabling early and individualized treatment.

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Figures

Figure 1

Figure 1

Standard curves for reconstituted biopsy samples.

Figure 2

Figure 2

Comparison of the procedural steps involved in the quantitation methods.

References

    1. Pruitt BA, Jr, McManus AT, Kim SH, Goodwin CW. Burn wound infections: current status. World J Surg. 1998;22:135–145. -PubMed
    1. Lee JJ, Marvin JA, Heimbach DM, Grube BJ, Engrave LH. Infection control in a burn center. J Burn Care Rehabil. 1992;11:575–580. -PubMed
    1. McManus AT, Mason AD, Jr, McManus WF, Pruitt BA., Jr Twenty-five year review of Pseudomonas aeruginosa bacteremia in a burn center. Eur J Clin Microbiol. 1985;4:219–223. -PubMed
    1. Teplitz C, Davis D, Mason AD, Jr, Moncrief JA. Pseudomonas burn wound sepsis. I. Pathogenesis of experimental Pseudomonas burn wound sepsis. J Surg Res. 1964;4:200–222. -PubMed
    1. Moncrief JA, Lindberg RB, Switzer WE, Pruitt BA., Jr Use of topical antibacterial therapy in the treatment of the burn wound. . Arch Surg. 1966;92:558–565. -PubMed

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