Universal GFP reporter for the study of vascular development - PubMed (original) (raw)
doi: 10.1002/1526-968x(200010)28:2<75::aid-gene50>3.0.co;2-s.
S Loughna, E Perens, B L Roman, W Liao, T C Chau, C D Richardson, T Kawate, J Kuno, B M Weinstein, D Y Stainier, T N Sato
Affiliations
- PMID: 11064424
- DOI: 10.1002/1526-968x(200010)28:2<75::aid-gene50>3.0.co;2-s
Universal GFP reporter for the study of vascular development
T Motoike et al. Genesis. 2000 Oct.
Abstract
We report the generation and characterization of transgenic mouse and zebrafish expressing green fluorescent protein (GFP) specifically in vascular endothelial cells in a relatively uniform fashion. These reporter lines exhibit fluorescent vessels in developing embryos and throughout adulthood, allowing visualization of the general vascular patterns with single cell resolution. Furthermore, we show the ability to purify endothelial cells from whole embryos and adult organs by a single step fluorescence activated cell sorting. We expect that these transgenic reporters will be useful tools for imaging vascular morphogenesis, global gene expression profile analysis of endothelial cells, and high throughput screening for vascular mutations.
Copyright 2000 Wiley-Liss, Inc.
Similar articles
- A fluorescent Tie1 reporter allows monitoring of vascular development and endothelial cell isolation from transgenic mouse embryos.
Iljin K, Petrova TV, Veikkola T, Kumar V, Poutanen M, Alitalo K. Iljin K, et al. FASEB J. 2002 Nov;16(13):1764-74. doi: 10.1096/fj.01-1043com. FASEB J. 2002. PMID: 12409319 - A mutant receptor tyrosine phosphatase, CD148, causes defects in vascular development.
Takahashi T, Takahashi K, St John PL, Fleming PA, Tomemori T, Watanabe T, Abrahamson DR, Drake CJ, Shirasawa T, Daniel TO. Takahashi T, et al. Mol Cell Biol. 2003 Mar;23(5):1817-31. doi: 10.1128/MCB.23.5.1817-1831.2003. Mol Cell Biol. 2003. PMID: 12588999 Free PMC article. - GATA-1 expression pattern can be recapitulated in living transgenic zebrafish using GFP reporter gene.
Long Q, Meng A, Wang H, Jessen JR, Farrell MJ, Lin S. Long Q, et al. Development. 1997 Oct;124(20):4105-11. doi: 10.1242/dev.124.20.4105. Development. 1997. PMID: 9374406 - The color of mice: in the light of GFP-variant reporters.
Hadjantonakis AK, Nagy A. Hadjantonakis AK, et al. Histochem Cell Biol. 2001 Jan;115(1):49-58. doi: 10.1007/s004180000233. Histochem Cell Biol. 2001. PMID: 11219608 Review. - GFP transgenic animals in biomedical research: a review of potential disadvantages.
Lipták N, Bősze Z, Hiripi L. Lipták N, et al. Physiol Res. 2019 Aug 29;68(4):525-530. doi: 10.33549/physiolres.934227. Epub 2019 Jul 25. Physiol Res. 2019. PMID: 31342754 Review.
Cited by
- Characteristics of blood-brain barrier heterogeneity between brain regions revealed by profiling vascular and perivascular cells.
Pfau SJ, Langen UH, Fisher TM, Prakash I, Nagpurwala F, Lozoya RA, Lee WA, Wu Z, Gu C. Pfau SJ, et al. Nat Neurosci. 2024 Oct;27(10):1892-1903. doi: 10.1038/s41593-024-01743-y. Epub 2024 Aug 29. Nat Neurosci. 2024. PMID: 39210068 Free PMC article. - Cysteine-rich with EGF-like domains 2 (CRELD2) is an endoplasmic reticulum stress-inducible angiogenic growth factor promoting ischemic heart repair.
Wu X, Zheng L, Reboll MR, Hyde LF, Mass E, Niessen HW, Kosanke M, Pich A, Giannitsis E, Tillmanns J, Bauersachs J, Heineke J, Wang Y, Korf-Klingebiel M, Polten F, Wollert KC. Wu X, et al. Nat Cardiovasc Res. 2024 Feb;3(2):186-202. doi: 10.1038/s44161-023-00411-x. Epub 2024 Jan 17. Nat Cardiovasc Res. 2024. PMID: 39196188 Free PMC article. - Immunolabeling-compatible PEGASOS tissue clearing for high-resolution whole mouse brain imaging.
Gao P, Rivera M, Lin X, Holmes TC, Zhao H, Xu X. Gao P, et al. Front Neural Circuits. 2024 Apr 17;18:1345692. doi: 10.3389/fncir.2024.1345692. eCollection 2024. Front Neural Circuits. 2024. PMID: 38694272 Free PMC article. - Experimental and theoretical model of microvascular network remodeling and blood flow redistribution following minimally invasive microvessel laser ablation.
Gruionu G, Baish J, McMahon S, Blauvelt D, Gruionu LG, Lenco MO, Vakoc BJ, Padera TP, Munn LL. Gruionu G, et al. Sci Rep. 2024 Apr 16;14(1):8767. doi: 10.1038/s41598-024-59296-w. Sci Rep. 2024. PMID: 38627467 Free PMC article. - Experimental and Theoretical Model of Single Vessel Minimally Invasive Micro-Laser Ablation: Inducing Microvascular Network Remodeling and Blood Flow Redistribution Without Compromising Host Tissue Function.
Gruionu G, Baish J, McMahon S, Blauvelt D, Gruionu LG, Lenco MO, Vakoc BJ, Padera TP, Munn LL. Gruionu G, et al. Res Sq [Preprint]. 2023 Dec 18:rs.3.rs-3754775. doi: 10.21203/rs.3.rs-3754775/v1. Res Sq. 2023. PMID: 38196660 Free PMC article. Preprint.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials