Immature CD4+CD8+ thymocytes do not polarize lipid rafts in response to TCR-mediated signals - PubMed (original) (raw)
Immature CD4+CD8+ thymocytes do not polarize lipid rafts in response to TCR-mediated signals
P J Ebert et al. J Immunol. 2000.
Abstract
TCR-mediated stimulation induces activation and proliferation of mature T cells. When accompanied by signals through the costimulatory receptor CD28, TCR signals also result in the recruitment of cholesterol- and glycosphingolipid-rich membrane microdomains (lipid rafts), which are known to contain several molecules important for T cell signaling. Interestingly, immature CD4(+)CD8(+) thymocytes respond to TCR/CD28 costimulation not by proliferating, but by dying. In this study, we report that, although CD4(+)CD8(+) thymocytes polarize their actin cytoskeleton, they fail to recruit lipid rafts to the site of TCR/CD28 costimulation. We show that coupling of lipid raft mobilization to cytoskeletal reorganization can be mediated by phosphoinositide 3-kinase, and discuss the relevance of these findings to the interpretation of TCR signals by immature vs mature T cells.
Similar articles
- Maturation versus death of developing double-positive thymocytes reflects competing effects on Bcl-2 expression and can be regulated by the intensity of CD28 costimulation.
McKean DJ, Huntoon CJ, Bell MP, Tai X, Sharrow S, Hedin KE, Conley A, Singer A. McKean DJ, et al. J Immunol. 2001 Mar 1;166(5):3468-75. doi: 10.4049/jimmunol.166.5.3468. J Immunol. 2001. PMID: 11207305 - CD5 costimulation up-regulates the signaling to extracellular signal-regulated kinase activation in CD4+CD8+ thymocytes and supports their differentiation to the CD4 lineage.
Zhou XY, Yashiro-Ohtani Y, Toyo-Oka K, Park CS, Tai XG, Hamaoka T, Fujiwara H. Zhou XY, et al. J Immunol. 2000 Feb 1;164(3):1260-8. doi: 10.4049/jimmunol.164.3.1260. J Immunol. 2000. PMID: 10640739 - TCR and Notch signaling in CD4 and CD8 T-cell development.
Laky K, Fleischacker C, Fowlkes BJ. Laky K, et al. Immunol Rev. 2006 Feb;209:274-83. doi: 10.1111/j.0105-2896.2006.00358.x. Immunol Rev. 2006. PMID: 16448548 Review. - Two receptors, two kinases, and T cell lineage determination.
Alarcón B, van Santen HM. Alarcón B, et al. Sci Signal. 2010 Mar 23;3(114):pe11. doi: 10.1126/scisignal.3114pe11. Sci Signal. 2010. PMID: 20332426 Review.
Cited by
- 'Nur'turing tumor T cell tolerance and exhaustion: novel function for Nuclear Receptor Nur77 in immunity.
Lith SC, van Os BW, Seijkens TTP, de Vries CJM. Lith SC, et al. Eur J Immunol. 2020 Nov;50(11):1643-1652. doi: 10.1002/eji.202048869. Epub 2020 Oct 25. Eur J Immunol. 2020. PMID: 33063848 Free PMC article. Review. - Increased membrane cholesterol in lymphocytes diverts T-cells toward an inflammatory response.
Surls J, Nazarov-Stoica C, Kehl M, Olsen C, Casares S, Brumeanu TD. Surls J, et al. PLoS One. 2012;7(6):e38733. doi: 10.1371/journal.pone.0038733. Epub 2012 Jun 19. PLoS One. 2012. PMID: 22723880 Free PMC article. - Functional development of the T cell receptor for antigen.
Ebert PJ, Li QJ, Huppa JB, Davis MM. Ebert PJ, et al. Prog Mol Biol Transl Sci. 2010;92:65-100. doi: 10.1016/S1877-1173(10)92004-8. Prog Mol Biol Transl Sci. 2010. PMID: 20800817 Free PMC article. - c-Cbl and Cbl-b ubiquitin ligases: substrate diversity and the negative regulation of signalling responses.
Thien CB, Langdon WY. Thien CB, et al. Biochem J. 2005 Oct 15;391(Pt 2):153-66. doi: 10.1042/BJ20050892. Biochem J. 2005. PMID: 16212556 Free PMC article. Review. - Measles virus interacts with and alters signal transduction in T-cell lipid rafts.
Avota E, Müller N, Klett M, Schneider-Schaulies S. Avota E, et al. J Virol. 2004 Sep;78(17):9552-9. doi: 10.1128/JVI.78.17.9552-9559.2004. J Virol. 2004. PMID: 15308747 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous