Transgene and transposon silencing in Chlamydomonas reinhardtii by a DEAH-box RNA helicase - PubMed (original) (raw)

Comment

Transgene and transposon silencing in Chlamydomonas reinhardtii by a DEAH-box RNA helicase

D Wu-Scharf et al. Science. 2000.

Abstract

The molecular mechanism(s) responsible for posttranscriptional gene silencing and RNA interference remain poorly understood. We have cloned a gene (Mut6) from the unicellular green alga Chlamydomonas reinhardtii that is required for the silencing of a transgene and two transposon families. Mut6 encodes a protein that is highly homologous to RNA helicases of the DEAH-box family. This protein is necessary for the degradation of certain aberrant RNAs, such as improperly processed transcripts, which are often produced by transposons and some transgenes.

PubMed Disclaimer

Similar articles

• Molecular biology. Unwinding RNA silencing.
  Baulcombe DC. Baulcombe DC. Science. 2000 Nov 10;290(5494):1108-9. doi: 10.1126/science.290.5494.1108. Science. 2000. PMID: 11185008
• Untemplated oligoadenylation promotes degradation of RISC-cleaved transcripts.
  Ibrahim F, Rohr J, Jeong WJ, Hesson J, Cerutti H. Ibrahim F, et al. Science. 2006 Dec 22;314(5807):1893. doi: 10.1126/science.1135268. Science. 2006. PMID: 17185594
• Diversification of the core RNA interference machinery in Chlamydomonas reinhardtii and the role of DCL1 in transposon silencing.
  Casas-Mollano JA, Rohr J, Kim EJ, Balassa E, van Dijk K, Cerutti H. Casas-Mollano JA, et al. Genetics. 2008 May;179(1):69-81. doi: 10.1534/genetics.107.086546. Genetics. 2008. PMID: 18493041 Free PMC article.
• The DEAD-box protein family of RNA helicases.
  Cordin O, Banroques J, Tanner NK, Linder P. Cordin O, et al. Gene. 2006 Feb 15;367:17-37. doi: 10.1016/j.gene.2005.10.019. Epub 2005 Dec 7. Gene. 2006. PMID: 16337753 Review.
• DExD/H-box proteins and their partners: helping RNA helicases unwind.
  Silverman E, Edwalds-Gilbert G, Lin RJ. Silverman E, et al. Gene. 2003 Jul 17;312:1-16. doi: 10.1016/s0378-1119(03)00626-7. Gene. 2003. PMID: 12909336 Review.

Cited by

• Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems.
  Caplen NJ, Parrish S, Imani F, Fire A, Morgan RA. Caplen NJ, et al. Proc Natl Acad Sci U S A. 2001 Aug 14;98(17):9742-7. doi: 10.1073/pnas.171251798. Epub 2001 Jul 31. Proc Natl Acad Sci U S A. 2001. PMID: 11481446 Free PMC article.
• aubergine mutations in Drosophila melanogaster impair P cytotype determination by telomeric P elements inserted in heterochromatin.
  Reiss D, Josse T, Anxolabéhère D, Ronsseray S. Reiss D, et al. Mol Genet Genomics. 2004 Oct;272(3):336-43. doi: 10.1007/s00438-004-1061-1. Epub 2004 Sep 14. Mol Genet Genomics. 2004. PMID: 15372228
• Chlamydomonas as a model for biofuels and bio-products production.
  Scranton MA, Ostrand JT, Fields FJ, Mayfield SP. Scranton MA, et al. Plant J. 2015 May;82(3):523-531. doi: 10.1111/tpj.12780. Epub 2015 Feb 18. Plant J. 2015. PMID: 25641390 Free PMC article. Review.
• RNA interference: more than a research tool in the vertebrates' adaptive immunity.
  Mak J. Mak J. Retrovirology. 2005 May 25;2:35. doi: 10.1186/1742-4690-2-35. Retrovirology. 2005. PMID: 15916707 Free PMC article.
• The rest is silence.
  Bernstein E, Denli AM, Hannon GJ. Bernstein E, et al. RNA. 2001 Nov;7(11):1509-21. RNA. 2001. PMID: 11720281 Free PMC article. Review.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Ovid Technologies, Inc.

• Other Literature Sources

  • The Lens - Patent Citations Database