The Isw2 chromatin remodeling complex represses early meiotic genes upon recruitment by Ume6p - PubMed (original) (raw)
The Isw2 chromatin remodeling complex represses early meiotic genes upon recruitment by Ume6p
J P Goldmark et al. Cell. 2000.
Free article
Abstract
The ISWI class of chromatin remodeling factors exhibits potent chromatin remodeling activities in vitro. However, the in vivo functions of this class of factors are unknown at a molecular level. We have found that S. cerevisiae Isw2 complex represses transcription of early meiotic genes during mitotic growth in a parallel pathway to Rpd3-Sin3 histone deacetylase complex. This repressor function of lsw2 complex is largely dependent upon Ume6p, which recruits the complex to target genes. Nuclease digestion analyses revealed that lsw2 complex establishes nuclease-inaccessible chromatin structure near the Ume6p binding site in vivo. Based on these findings, we propose a model for the mechanism of transcriptional repression by two distinct chromatin remodeling complexes.
Similar articles
- Widespread collaboration of Isw2 and Sin3-Rpd3 chromatin remodeling complexes in transcriptional repression.
Fazzio TG, Kooperberg C, Goldmark JP, Neal C, Basom R, Delrow J, Tsukiyama T. Fazzio TG, et al. Mol Cell Biol. 2001 Oct;21(19):6450-60. doi: 10.1128/MCB.21.19.6450-6460.2001. Mol Cell Biol. 2001. PMID: 11533234 Free PMC article. - CLN1 and its repression by Xbp1 are important for efficient sporulation in budding yeast.
Mai B, Breeden L. Mai B, et al. Mol Cell Biol. 2000 Jan;20(2):478-87. doi: 10.1128/MCB.20.2.478-487.2000. Mol Cell Biol. 2000. PMID: 10611226 Free PMC article. - Acetylation of the transcriptional repressor Ume6p allows efficient promoter release and timely induction of the meiotic transient transcription program in yeast.
Law MJ, Mallory MJ, Dunbrack RL Jr, Strich R. Law MJ, et al. Mol Cell Biol. 2014 Feb;34(4):631-42. doi: 10.1128/MCB.00256-13. Epub 2013 Dec 2. Mol Cell Biol. 2014. PMID: 24298021 Free PMC article. - Histone deacetylase: a regulator of transcription.
Wolffe AP. Wolffe AP. Science. 1996 Apr 19;272(5260):371-2. doi: 10.1126/science.272.5260.371. Science. 1996. PMID: 8602525 Review. No abstract available. - Transcriptional regulation of meiosis in budding yeast.
Kassir Y, Adir N, Boger-Nadjar E, Raviv NG, Rubin-Bejerano I, Sagee S, Shenhar G. Kassir Y, et al. Int Rev Cytol. 2003;224:111-71. doi: 10.1016/s0074-7696(05)24004-4. Int Rev Cytol. 2003. PMID: 12722950 Review.
Cited by
- The transcriptional regulator Ume6 is a major driver of early gene expression during gametogenesis.
Harris A, Ünal E. Harris A, et al. Genetics. 2023 Oct 4;225(2):iyad123. doi: 10.1093/genetics/iyad123. Genetics. 2023. PMID: 37431893 Free PMC article. - The Dynamics of Adaptation to Stress from Standing Genetic Variation and de novo Mutations.
Ament-Velásquez SL, Gilchrist C, Rêgo A, Bendixsen DP, Brice C, Grosse-Sommer JM, Rafati N, Stelkens R. Ament-Velásquez SL, et al. Mol Biol Evol. 2022 Nov 3;39(11):msac242. doi: 10.1093/molbev/msac242. Mol Biol Evol. 2022. PMID: 36334099 Free PMC article. - Partitioned usage of chromatin remodelers by nucleosome-displacing factors.
Chen H, Kharerin H, Dhasarathy A, Kladde M, Bai L. Chen H, et al. Cell Rep. 2022 Aug 23;40(8):111250. doi: 10.1016/j.celrep.2022.111250. Cell Rep. 2022. PMID: 36001970 Free PMC article. - UME6 Is Involved in the Suppression of Basal Transcription of ABC Transporters and Drug Resistance in the ρ+ Cells of Saccharomyces cerevisiae.
Yamada Y. Yamada Y. Microorganisms. 2022 Mar 10;10(3):601. doi: 10.3390/microorganisms10030601. Microorganisms. 2022. PMID: 35336175 Free PMC article. - The ACF chromatin-remodeling complex is essential for Polycomb repression.
Wiles ET, Mumford CC, McNaught KJ, Tanizawa H, Selker EU. Wiles ET, et al. Elife. 2022 Mar 8;11:e77595. doi: 10.7554/eLife.77595. Elife. 2022. PMID: 35257662 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases