Heat shock treatment enhances graft cell survival in skeletal myoblast transplantation to the heart - PubMed (original) (raw)
Heat shock treatment enhances graft cell survival in skeletal myoblast transplantation to the heart
K Suzuki et al. Circulation. 2000.
Abstract
Background: Graft survival after skeletal myoblast transplantation is affected by various pathological processes caused by environmental stress. Heat shock is known to afford protection of several aspects of cell metabolism and function. We hypothesized that prior heat shock treatment of graft cells would improve their survival after cell transplantation.
Methods and results: L6 rat skeletal myoblasts expressing ss-galactosidase (ss-gal) were subjected to heat shock (42 degrees C, 1 hour). Increased expression of heat shock protein 72 was detected 24 hours later in the heat-shocked cells. After hypoxia-reoxygenation in vitro, lactate dehydrogenase leakage was significantly attenuated in the heat-shocked cells; in addition, the percentage of early apoptosis was lower in this group measured by flow cytometry with annexin V staining. For the in vivo study, 1 x 10(6) heat-shocked (hsCTx) or normal-cultured (CTx) myoblasts were infused into the explanted rat hearts through the coronary artery followed by heterotopic heart transplantation. ss-gal activity was significantly higher in the hsCTx group after cell transplantation, with an estimated 8 x 10(6) surviving cells per heart in the hsCTx group and 5 x 10(6) cells in the CTx group on day 28. Discrete loci of grafted cells were globally observed in the myocardium of the hsCTx and CTx groups, with a higher frequency in the hsCTx group. Surviving myoblasts occasionally differentiated into myotubes and had integrated with the native cardiomyocytes.
Conclusions: Heat-shocked skeletal myoblasts demonstrated improved tolerance to hypoxia-reoxygenation insult in vitro and enhanced survival when grafted into the heart. Heat shock treatment could be useful in improving graft cell survival in cell transplantation.
Similar articles
- Development of a novel method for cell transplantation through the coronary artery.
Suzuki K, Brand NJ, Smolenski RT, Jayakumar J, Murtuza B, Yacoub MH. Suzuki K, et al. Circulation. 2000 Nov 7;102(19 Suppl 3):III359-64. doi: 10.1161/01.cir.102.suppl_3.iii-359. Circulation. 2000. PMID: 11082414 - Heat shock improves recovery and provides protection against global ischemia after hypothermic storage.
Gowda A, Yang C, Asimakis GK, Rastegar S, Motamedi M. Gowda A, et al. Ann Thorac Surg. 1998 Dec;66(6):1991-7. doi: 10.1016/s0003-4975(98)00905-9. Ann Thorac Surg. 1998. PMID: 9930482 - Heat shock pretreatment enhances porcine myoblasts survival after autotransplantation in intact skeletal muscle.
Yang S, Laumonier T, Menetrey J. Yang S, et al. Sci China C Life Sci. 2007 Aug;50(4):438-46. doi: 10.1007/s11427-007-0065-6. Sci China C Life Sci. 2007. PMID: 17653663 Retracted. - Heat shock attenuates VEGF expression in three-dimensional myoblast sheets deteriorating therapeutic efficacy in heart failure.
Augustin M, Ali Asim Mahar M, Lakkisto P, Tikkanen I, Vento A, Patila T, Harjula A. Augustin M, et al. Med Sci Monit. 2011 Dec;17(12):BR345-53. doi: 10.12659/msm.882120. Med Sci Monit. 2011. PMID: 22129892 Free PMC article. - Myocyte transplantation for myocardial repair: a few good cells can mend a broken heart.
El Oakley RM, Ooi OC, Bongso A, Yacoub MH. El Oakley RM, et al. Ann Thorac Surg. 2001 May;71(5):1724-33. doi: 10.1016/s0003-4975(00)02596-0. Ann Thorac Surg. 2001. PMID: 11383847 Review.
Cited by
- Preconditioning of Human Skeletal Myoblast with Stromal Cell-derived Factor-1α Promotes Cytoprotective Effects against Oxidative and Anoxic Stress.
Elmadbouh I, Haider HKh, Ashraf M, Chachques JC. Elmadbouh I, et al. Int J Stem Cells. 2011 Jun;4(1):50-60. doi: 10.15283/ijsc.2011.4.1.50. Int J Stem Cells. 2011. PMID: 24298334 Free PMC article. - Diazoxide protects L6 skeletal myoblasts from H2O2-induced apoptosis via the phosphatidylinositol-3 kinase/Akt pathway.
Chen W, Liu Y, Xue G, Zhang L, Zhang L, Shao S. Chen W, et al. Inflamm Res. 2016 Jan;65(1):53-60. doi: 10.1007/s00011-015-0890-1. Epub 2015 Nov 2. Inflamm Res. 2016. PMID: 26525360 - Embryonic stem cells in cardiac repair and regeneration.
Singla DK. Singla DK. Antioxid Redox Signal. 2009 Aug;11(8):1857-63. doi: 10.1089/ars.2009.2491. Antioxid Redox Signal. 2009. PMID: 19203218 Free PMC article. Review. - Heat shock pretreatment improves stem cell repair following ischemia-reperfusion injury via autophagy.
Qiao PF, Yao L, Zhang XC, Li GD, Wu DQ. Qiao PF, et al. World J Gastroenterol. 2015 Dec 7;21(45):12822-34. doi: 10.3748/wjg.v21.i45.12822. World J Gastroenterol. 2015. PMID: 26668506 Free PMC article. - The Current Dilemma and Breakthrough of Stem Cell Therapy in Ischemic Heart Disease.
Liu C, Han D, Liang P, Li Y, Cao F. Liu C, et al. Front Cell Dev Biol. 2021 Apr 22;9:636136. doi: 10.3389/fcell.2021.636136. eCollection 2021. Front Cell Dev Biol. 2021. PMID: 33968924 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources