Role of dynamin, Src, and Ras in the protein kinase C-mediated activation of ERK by gonadotropin-releasing hormone - PubMed (original) (raw)
. 2001 Feb 16;276(7):4554-63.
doi: 10.1074/jbc.M006995200. Epub 2000 Nov 16.
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- PMID: 11083862
- DOI: 10.1074/jbc.M006995200
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Role of dynamin, Src, and Ras in the protein kinase C-mediated activation of ERK by gonadotropin-releasing hormone
O Benard et al. J Biol Chem. 2001.
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- Role of dynamin, Src, and Ras in the protein kinase C-mediated activation of ERK by gonadotropin-releasing hormone.
Benard O, Naor Z, Seger R. Benard O, et al. J Biol Chem. 2017 May 26;292(21):8855. doi: 10.1074/jbc.A117.006995. J Biol Chem. 2017. PMID: 28550141 Free PMC article. No abstract available.
Abstract
G-protein-coupled receptors are a large group of integral membranal receptors, which in response to ligand binding initiate diverse downstream signaling. Here we studied the gonadotropin-releasing hormone (GnRH) receptor, which uses Gq for its downstream signaling. We show that extracellular signal-regulated kinase (ERK) activation is fully dependent on protein kinase C (PKC), but only partially dependent on Src, dynamin, and Ras. Receptor tyrosine kinases, FAK, Gbetagamma, and beta-arrestin, which were implicated in some G-protein-coupled receptor signaling to MAPK cascades, do not play a role in the GnRH to ERK pathway. Our results suggest that the activation of ERK by GnRH involves two distinct signaling pathways, which converge at the level of Raf-1. The main pathway involves a direct activation of Raf-1 by PKC, and this step is partially dependent on a second pathway consisting of Ras activation, which occurs in a dynamin-dependent manner, downstream of Src.
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