Use of a TT virus ORF1 recombinant protein to detect anti-TT virus antibodies in human sera - PubMed (original) (raw)
Use of a TT virus ORF1 recombinant protein to detect anti-TT virus antibodies in human sera
Catherine Ott et al. J Gen Virol. 2000 Dec.
Abstract
TT virus (TTV), isolated initially from a Japanese patient with hepatitis of unknown aetiology, has since been found to infect both healthy and diseased individuals and numerous prevalence studies have raised questions about its role in unexplained hepatitis. In order to determine the prevalence of ongoing TTV infection as well as resolved infection, a serological study was performed with a recombinant protein generated from the open reading frame 1 (ORF1) sequence isolated from a French patient infected by TTV. The C-terminal end of the ORF1 protein, containing a particularly hydrophilic region, was retained to be used as antigen to detect the presence of anti-TTV antibodies in serum samples by a Western blot analysis. For this purpose, the C-terminal ORF1 region was expressed in fusion with a hexahistidine tail in E. coli and purified by metal-chelate affinity chromatography. The serological screening of 70 human sera, including 30 patients with hepatitis of unknown aetiology, 30 blood donors and 10 healthy children, allowed the immune response of infected hosts to be identified by the detection of TTV-specific antibodies, with a very high prevalence of 98.6% in the human sera tested. In contrast, TTV DNA was detected by PCR in only 76.1% of the tested sera. The use of the ORF1 C-terminal recombinant protein thereby provided a diagnostic tool to follow the immune response of the host against TTV.
Similar articles
- Expression of all six human Torque teno virus (TTV) proteins in bacteria and in insect cells, and analysis of their IgG responses.
Kakkola L, Bondén H, Hedman L, Kivi N, Moisala S, Julin J, Ylä-Liedenpohja J, Miettinen S, Kantola K, Hedman K, Söderlund-Venermo M. Kakkola L, et al. Virology. 2008 Dec 20;382(2):182-9. doi: 10.1016/j.virol.2008.09.012. Epub 2008 Oct 22. Virology. 2008. PMID: 18947848 - Cloning and expression of N22 region of Torque Teno virus (TTV) genome and use of peptide in developing immunoassay for TTV antibodies.
Mankotia DS, Irshad M. Mankotia DS, et al. Virol J. 2014 May 20;11:96. doi: 10.1186/1743-422X-11-96. Virol J. 2014. PMID: 24884576 Free PMC article. - Development of an Immunoassay for Detection of Torque Teno Virus (TTV) Antibodies Using the N22 Expression Product from TTV Genotype 2.
Mankotia DS, Irshad M. Mankotia DS, et al. Intervirology. 2017;60(5):207-216. doi: 10.1159/000487481. Epub 2018 Mar 27. Intervirology. 2017. PMID: 29587272 - Torque teno virus in liver diseases: On the way towards unity of view.
Reshetnyak VI, Maev IV, Burmistrov AI, Chekmazov IA, Karlovich TI. Reshetnyak VI, et al. World J Gastroenterol. 2020 Apr 21;26(15):1691-1707. doi: 10.3748/wjg.v26.i15.1691. World J Gastroenterol. 2020. PMID: 32351287 Free PMC article. Review. - Molecular properties, biology, and clinical implications of TT virus, a recently identified widespread infectious agent of humans.
Bendinelli M, Pistello M, Maggi F, Fornai C, Freer G, Vatteroni ML. Bendinelli M, et al. Clin Microbiol Rev. 2001 Jan;14(1):98-113. doi: 10.1128/CMR.14.1.98-113.2001. Clin Microbiol Rev. 2001. PMID: 11148004 Free PMC article. Review.
Cited by
- Anelloviruses versus human immunity: how do we control these viruses?
Timmerman AL, Schönert ALM, van der Hoek L. Timmerman AL, et al. FEMS Microbiol Rev. 2024 Jan 12;48(1):fuae005. doi: 10.1093/femsre/fuae005. FEMS Microbiol Rev. 2024. PMID: 38337179 Free PMC article. Review. - The Impact of First-Time SARS-CoV-2 Infection on Human Anelloviruses.
Timmerman AL, Commandeur L, Deijs M, Burggraaff MGJM, Lavell AHA, van der Straten K, Tejjani K, van Rijswijk J, van Gils MJ, Sikkens JJ, Bomers MK, van der Hoek L. Timmerman AL, et al. Viruses. 2024 Jan 9;16(1):99. doi: 10.3390/v16010099. Viruses. 2024. PMID: 38257799 Free PMC article. - Psychrobacter Phage Encoding an Antibiotics Resistance Gene Represents a Novel Caudoviral Family.
Wang H, Ren L, Liang Y, Zheng K, Guo R, Liu Y, Wang Z, Han Y, Zhang X, Shao H, Sung YY, Mok WJ, Wong LL, McMinn A, Wang M. Wang H, et al. Microbiol Spectr. 2023 Aug 17;11(4):e0533522. doi: 10.1128/spectrum.05335-22. Epub 2023 Jun 5. Microbiol Spectr. 2023. PMID: 37272818 Free PMC article. - Harmless or Threatening? Interpreting the Results of Molecular Diagnosis in the Context of Virus-Host Relationships.
Abade Dos Santos FA, Portela SJ, Nogueira T, Carvalho CL, de Sousa R, Duarte MD. Abade Dos Santos FA, et al. Front Microbiol. 2021 May 21;12:647730. doi: 10.3389/fmicb.2021.647730. eCollection 2021. Front Microbiol. 2021. PMID: 34093464 Free PMC article. Review. - Prevalence of torque teno virus in healthy individuals and those infected with hepatitis C virus living in Yazd, Iran.
Moghimi M, Shayestehpour M, Doosti M, Ahmadi Vasmehjani A, Seyed Khorrami SM, Ahmadi AS, Akhondi-Meybodi M. Moghimi M, et al. Caspian J Intern Med. 2020;11(2):199-204. doi: 10.22088/cjim.11.2.199. Caspian J Intern Med. 2020. PMID: 32509249 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources